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    mouse-liver-tissue-lysate-0-days-ab7264.pdf

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Kits/ Lysates/ Other Lysates Tissue Lysates Mouse Other
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Mouse liver tissue lysate (0 days) (ab7264)

  • Datasheet
  • SDS
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Abpromise

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Overview

  • Product name

    Mouse liver tissue lysate (0 days)
    See all Liver lysates
  • General notes

    Source: Outbred NIH-Swiss Albino mice (0 days old)


    The mouse liver tissue was collected from zero (0) day-old mice. The lysate was prepared by homogenization in mo dified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties

  • Mycoplasma free

    Yes
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituents: 60.05% Water, 12.5% Glycerol (glycerin, glycerine), 9% Tris HCl, 7.7% DTT, 4.4% Sodium chloride, 1% Triton-X-100, 1% Sodium deoxycholate, 1.1% Sodium lauryl sulfate, 0.15% EDTA disodium salt, 0.5% Aprotinin, 0.5% Leupeptin hemisulfate, 0.09% PMSF, 0.01% Bromophenol blue
  • Research areas

    • Kits/ Lysates/ Other
    • Lysates
    • Tissue Lysates
    • Mouse
    • Other
    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Mouse
    • Gastrointestinal

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Mouse liver tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.
Notes
WB
Use at an assay dependent concentration. Mouse liver tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab7264? Please let us know so that we can cite the reference in this datasheet.

    ab7264 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Inquiry: Hi, I have some anti-scavenger BI + BII antibody (ab36970) and I want to prepare a positive control to use with it. On the data sheet total mouse liver lysate was used. Could you tell me how this liver lysate was prepared? Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Jul 11 2012

    Answer

    Thank you for your enquiry

    We obtain this product from an external source and unfortunately they are not able to provide specific information about the sample preparation.

    I reviewed the literature and found the following protocol that you may find useful:

    1. Used 1-2 liver lobes.
    2. Homogenize tissue in PBS/Protease Inhibitor cocktail.
    3. After centrifugation resuspend the pellet in approx. 400µl RIPA lysis buffer 30min (vortex every 5-10 min).
    4. Centrifuge to give whole cell extracts.
    5. To improve the amount of sample extracted, repeat this lysis step with the pellet from the centrifugation and pool the supernatant of the 1st and the 2nd lysis.

    We also stock a range of liver lysates:

    Liver (Mouse) Whole Cell Lysate - normal tissue, 0 days old
    www.abcam.com/ab7264

    Liver (Mouse) Whole Cell Lysate - normal tissue, 7 days old
    www.abcam.com/ab7266

    Liver (Mouse) Whole Cell Lysate - normal tissue
    www.abcam.com/ab29301

    Liver (Human) Tissue Lysate - adult normal tissue
    www.abcam.com/ab29889

    Please let me know if there is any more information you need.

    Read More

    Abcam Scientific Support

    Answered on Jul 11 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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