Mouse liver tissue lysate - mitochondrial extract (ab110343)

Overview

  • Product name
    Mouse liver tissue lysate - mitochondrial extract
    See all Mouse Liver lysates
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    50 µg of mouse liver mitochondria solubilized in SDS-PAGE loading buffer and supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.

  • Tested applications
    Suitable for: SDS-PAGE, WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Constituent: 99% SDS-PAGE Sample Buffer
  • Concentration information loading...
  • Lysate notes
    50 µg of mouse liver mitochondria solubilized in SDS-PAGE loading buffer and supplemented with reducing agent. This sample is ready for SDS-electrophoresis and acts as a positive control in Western blotting applications.

Applications

Our Abpromise guarantee covers the use of ab110343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
SDS-PAGE Use at an assay dependent dilution.
WB Use at an assay dependent dilution. 10.0 µg/lane

References

ab110343 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry. This ab110343  Mouse Liver Mitochondrial lysate was prepared according to our standard mitochondrial isolation: Dounce homogenization of tissue in an iso-osmotic buffer followed by differential centrifugation to enrich for mitochondria.  The preparation contains mitochondrial membranes and matrix enzymes however since it has been frozen the membranes are less intact and therefore uncoupled. I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.  

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