Question (26668) | Mouse liver tissue lysate - mitochondrial extract (ab110349)

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Question

is this lysate and enriched enfraction or a purified fraction (pure mitochondria)? If this is purified how is the mitochondria isolated?

Answer

Thank you for your phone call yesterday and for your patience while I have been in touch with the lab scientists at MitoSciences. Following is their reply to your questions: "These mitochondria were prepared by our mitochondrial isolation kit: https://www.abcam.com/Mitochondria-Isolation-Kit-for-Tissue-with-Dounce-Homogenizer-ab110169.html The principal : homogenize cells in an iso-osmotic buffer, centrifuge several times at low speed to remove cell debris, and nuclei.  Then spin at precisely 12000 g to collect most of the mitochondria. The mitochondria are washed and pelleted several more times at 12000 g.  Finally the washed pellet is taken up in an iso-osmotic buffer and frozen. This does a good job of mitochondrial isolation and enrichment, but there may be impurities there. It is possible to make very pure mitochondria by floating them on a  gradient of some kind (e.g percoll) but yields are lower so we do not do this. " Please let me know if you have any further questions and I'll be happy to help.  

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