Mouse liver tissue lysate - total protein (7 days) (ab7266)

Overview

  • Product name
    Mouse liver tissue lysate - total protein (7 days)
    See all Liver lysates
  • Description
    Mouse liver total protein lysate
  • General notes
    Source: Outbred NIH-Swiss Albino mice (7 days old)


    The mouse liver tissue was collected from seven (7) day-old mice. The lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris -HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethylsulfonyl flouride, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris -HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • Tested applications
    Suitable for: WBmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab7266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Mouse liver tissue lysate is ready to load on SDS-PAGE for Western blotting. It is recommended to load 10 µg to 20 µg per lane for mini gel.

References

ab7266 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry

We obtain this product from an external source and unfortunately they are not able to provide specific information about the sample preparation.

I reviewed the literature and found the following protocol that you may find useful:

1. Used 1-2 liver lobes.
2. Homogenize tissue in PBS/Protease Inhibitor cocktail.
3. After centrifugation resuspend the pellet in approx. 400µl RIPA lysis buffer 30min (vortex every 5-10 min).
4. Centrifuge to give whole cell extracts.
5. To improve the amount of sample extracted, repeat this lysis step with the pellet from the centrifugation and pool the supernatant of the 1st and the 2nd lysis.

We also stock a range of liver lysates:

Liver (Mouse) Whole Cell Lysate - normal tissue, 0 days old
www.abcam.com/ab7264

Liver (Mouse) Whole Cell Lysate - normal tissue, 7 days old
www.abcam.com/ab7266

Liver (Mouse) Whole Cell Lysate - normal tissue
www.abcam.com/ab29301

Liver (Human) Tissue Lysate - adult normal tissue
www.abcam.com/ab29889

Please let me know if there is any more information you need.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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