Mouse Loading Control Antibody Panel (ab199713)
Overview
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Product name
Mouse Loading Control Antibody Panel
See all Loading Control Panel kits -
Product overview
Ab199713 is a sampler pack of loading control mouse antibodies and a Goat Anti-Mouse IgG secondary antibody conjugated to HRP.
This panel contains sample sizes of all mouse host primary antibodies against the following housekeeping targets: alpha Tubulin, GAPDH, COXIV, Histone H3, alpha 1 NaK ATPase.
The Mouse Loading Control Antibody Panel is designed for validation and confirmation of western blot analysis when tested in conjunction with your proteins of interest.
Please refer to each individual component datasheet for storage instructions.
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Refer to the 'Associated Products' section below to see HRP-conjugated versions of these antibodies.
Properties
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Storage instructions
Store at -80°C. Please refer to protocols.
Associated products
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Related Products
- HRP Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab196696)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
- HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)
- HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164)
- HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)
Images
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All lanes : Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040)
Observed band size: 50 kDa why is the actual band size different from the predicted?
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406 -
Lanes 1-5 : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) ((Blocked in 5% MILK))
Lanes 6-10 : Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484)
Lanes 1 & 6 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lanes 2 & 7 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lanes 3 & 8 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lanes 4 & 9 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lanes 5 & 10 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (ab7902)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Mouse (HRP conjugated) at 1/5000 dilution
Observed band size: 40 kDa why is the actual band size different from the predicted?
The membrane 1-5 was blocked in 5% Milk (1 hour). The membrane 6-10 was blocked in 5% BSA (1 hour). Abcam routinely uses 5% BSA to block however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent and significantly decreases the signal on the membrane. We have informed all users of the antibody of this finding. -
All lanes : Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab33985) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
Lane 4 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 5 : Kidney (Cow) Tissue Lysate (ab29073)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 15 kDa why is the actual band size different from the predicted? -
All lanes : Anti-Histone H3 antibody [mAbcam 24834] - Nuclear Loading Control and ChIP Grade (ab24834) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : Testis (Mouse) Tissue Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Observed band size: 18 kDa why is the actual band size different from the predicted? -
Lanes 1-3 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 1 µg/ml
Lanes 4-6 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 2 µg/ml
Lanes 7-9 : Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab7671) at 5 µg/ml
Lanes 1 & 7 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lanes 2 & 5 & 8 : Brain (Mouse) Tissue Lysate (ab27253)
Lanes 3 & 6 & 9 : Brain (Rat) Tissue Lysate (ab7942)
Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lysates/proteins at 20 µg per lane.
Observed band size: 112 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (183)
ab199713 has been referenced in 183 publications.
- Gabandé-Rodríguez E et al. High sphingomyelin levels induce lysosomal damage and autophagy dysfunction in Niemann Pick disease type A. Cell Death Differ N/A:N/A (2014). PubMed: 24488099
- Beaudry JL et al. Effects of selective and non-selective glucocorticoid receptor II antagonists on rapid-onset diabetes in young rats. PLoS One 9:e91248 (2014). PubMed: 24642683
- Konitsiotis AD et al. Attenuation of hedgehog acyltransferase-catalyzed sonic Hedgehog palmitoylation causes reduced signaling, proliferation and invasiveness of human carcinoma cells. PLoS One 9:e89899 (2014). PubMed: 24608521
- Uzer G et al. Gap junctional communication in osteocytes is amplified by low intensity vibrations in vitro. PLoS One 9:e90840 (2014). PubMed: 24614887
- Arroyo AI et al. Pharmacological reversion of sphingomyelin-induced dendritic spine anomalies in a Niemann Pick disease type A mouse model. EMBO Mol Med 6:398-413 (2014). PubMed: 24448491