Overview

  • Product name

    Mouse LOX1 ELISA Kit (OLR1)
    See all LOX 1 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Mouse serum 8 2.8%
    Inter-assay
    Sample n Mean SD CV%
    Mouse serum 3 8.9%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    7.9 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 87 86% - 88%
    Cell culture media 109 108% - 110%
    Heparin Plasma 106 99% - 111%
    EDTA Plasma 108 100% - 113%
    Citrate Plasma 113 103% - 119%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Goat, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s LOX1 (OLR1) mouse in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse LOX1 protein in serum, plasma, and cell culture supernatants.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Mouse Lectin like oxidized low density lipoprotein receptor 1 (LOX1), also known as oxidized low density lipoprotein receptor 1 (OLR-1), is a type II transmembrane receptor belonging to the C type lectin family. The mouse LOX1 gene encodes a 363 amino acid (aa) residue protein with a short N-terminal intracellular domain, a transmembrane domain, and a C-type lectin like domain which is required for ligand recognition. Mouse LOX1 has 57% and 79% identity to Human and Rat LOX1, respectively.

    LOX1 is a receptor that mediates the recognition, internalization, and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. The association of LOX1 with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, LOX1 acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Finally, LOX1 also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, and apoptotic cells.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse LOX1 Capture Antibody 1 x 600µl
    10X Mouse LOX1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Mouse LOX1Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.
  • Tissue specificity

    Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.
  • Involvement in disease

    Note=Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility.
    Note=OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Domain

    The cytoplasmic region is required for subcellular sorting on the cell surface.
    The C-type lectin domain mediates the recognition and binding of oxLDL.
  • Post-translational
    modifications

    The intrachain disulfide-bonds prevent N-glycosylation at some sites.
    N-glycosylated.
  • Cellular localization

    Cell membrane. Secreted. A secreted form also exists.
  • Information by UniProt
  • Alternative names

    • C-type lectin domain family 8 member A
    • CLEC8A
    • hLOX 1
    • hLOX-1
    • Lectin like oxidized LDL receptor 1
    • Lectin like oxLDL receptor 1
    • Lectin type oxidized LDL receptor 1
    • Lectin-like oxidized LDL receptor 1
    • Lectin-like oxLDL receptor 1
    • Lectin-type oxidized LDL receptor 1
    • low density lipoprotein oxidized, receptor 1
    • LOX-1
    • LOXIN
    • Olr1
    • OLR1_HUMAN
    • Ox LDL receptor 1
    • Ox-LDL receptor 1
    • Oxidised low density lipoprotein (lectin like) receptor 1
    • Oxidized low density lipoprotein receptor 1
    • Oxidized low density lipoprotein receptor 1 soluble form
    • Oxidized low-density lipoprotein receptor 1
    • OxLDL receptor 1
    • SCARE1
    • Scavenger receptor class E, member 1
    • SLOX1
    • soluble form
    • SR-EI
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab204521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between mouse RBP4 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity with shorter protocol time.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Native mouse LOX1 was measured in 50% mouse serum and 50% mouse lung supernatants (lung cultured 6 days in RPMI + 10% Fetal Bovine Serum) diluted in a 2-fold dilution series in Sample Diluent NS. Recombinant mouse LOX1 was spiked into 10% cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse LOX1 were measured in duplicate and interpolated from the mouse LOX1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

  • Native mouse LOX1 was measured in 25% mouse plasma citrate, 100% mouse plasma EDTA, and 50% mouse plasma heparin diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse LOX1 were measured in duplicate and interpolated from the mouse LOX1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

  • Separate sections of mouse lung were cultured for either 5 or 6 days in RPMI + 10% Fetal bovine serum. The concentrations of mouse LOX1 were measured in duplicate in 25% mouse lung supernatants and interpolated from the mouse LOX1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

Protocols

References

ab204521 has not yet been referenced specifically in any publications.

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