Mouse lung normal tissue lysate - total protein (ab29297)

Q&A (4)

Overview

  • Product name
    Mouse lung normal tissue lysate - total protein
    See all Lung lysates
  • Description
    Mouse lung total protein lysate
  • General notes


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.

  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage buffer
    Preservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notes
    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab29297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

References

ab29297 has not yet been referenced specifically in any publications.

Publishing research using ab29297? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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4 Q&A

10
Votes: Highest First

would it be possible for us to get another vial of the ab81673 antibody as we talked about on Thursday? Our original purchase order number for the ab42885 was 1263787. We are going to try to immunoprecipitate VAP-1 from the lysate then detect it via we...

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Thanks for your reply.

ab81673 has been tested for use in WB and IP so the experiment you propose should be fine. An alternative is to use one VAP1 antibody for IP and second VAP1 antibody for WB.

I am sorry this product did not per...

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I'm sorry if this wasn't clear in the last email, but we actually used up the lysate sent to us when repeating the experiment trying to get it to work, so it would be impossible to repeat these tests without a new lysate. Our initial question was to fi...

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Thanks for your reply.

Perhaps it would be more efficient to discuss this issue on the phone. Please feel free to call me. Alternatively, if you provide a phone number I'm happy to give you a call.

I look forward to speaking with yo...

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I attached our previous run of the lysate you sent us next to one we had. The one we’ve been using still produces a 75 kDa band, rather than the 85-100 kDa band that your website lists as VAP-1. As you can see, the lysate you sent us produce...

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Thanks for your email.

Since you have ab81673 I would recommend using it in a western blot with the 2 lysates you have been testing. It is quite likely this antibody will work in western blotting as it has already been shown to work in a dive...

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It's been a little while since I emailed you, so to refresh your memory I'm Andrew Knapp from Dr. Pilar Alcaide's lab. We're moving back toward VAP-1 western blotting experiments, and we're again having issues with finding VAP-1 in lysate tissues....

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Thanks for your email.

The data you are seeing do appear unexpected.

Reducing conditions are used during western blotting.

Before I believe a loading control western blot was producing expected banding pattern so I don't t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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