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I'm sorry if this wasn't clear in the last email, but we actually used up the lysate sent to us when repeating the experiment trying to get it to work, so it would be impossible to repeat these tests without a new lysate. Our initial question was to find out if the 75 kDa band we see in our lysate is in fact VAP-1. You have reported on the website that the antibody ab42885 detects VAP-1 as an 85-100 kDa band in lung lysate. You sent us your lung lysate as a positive control, and we were not able to detect VAP-1. We need that control in order to interpret our studies. Can you help us with this?
Also, the antibody we've been using for FACS, ab81673, has not been reported or guaranteed to work in western blot. We bought the second antibody, (ab42885) specifically because it was guaranteed to detect VAP-1 in a western blot. If we can't get it to work, or if you all don't have a different suggestion for changing the protocol, would it be possible for us to get a credit for this antibody, or a second ab81673 for free? I know you've sent us some free products, which again I really appreciate, but at the end of the day, if we can't get them to work at all when we bought them for one specific purpose, I feel we should get a credit. I really hope you can work with us by giving us scientific support with this problem.
Asked on Aug 20 2012
Thanks for your reply.
Perhaps it would be more efficient to discuss this issue on the phone. Please feel free to call me. Alternatively, if you provide a phone number I'm happy to give you a call.
I look forward to speaking with you. I'm sure we will quickly come to an agreeable solution.
Answered on Aug 20 2012