Overview

  • Product name

    Mouse M-CSF ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 8 4.35%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 3 6.28%
  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    3.7 pg/ml
  • Range

    46.9 pg/ml - 3000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 97 92% - 105%
    Cell culture media 86 83% - 90%
    Citrate Plasma 110 107% - 112%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Rabbit, Goat, Hamster, Cow, Human
  • Product overview

    Abcam’s M-CSF Mouse in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of M-CSF protein in mouse serum, plasma (citrate), cell culture supernatants and cell extracts.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

    Sensitivity: 

    Samples diluted in Sample Diluent NS = 7.2 pg/mL

    Samples diluted in 1X Cell Extraction Buffer PTR = 3.7 pg/mL

  • Notes

    M-CSF is a secreted cytokine that plays an essential role in the regulation of differentiation and maintenance of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. It plays an important role in innate immunity and in inflammatory processes where it acts to promote the release of proinflammatory chemokines. M-CSF also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Reports show that it is required for normal male and female fertility. It also acts in reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse M-CSF Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 4BI 1 x 6ml
    Mouse M-CSF Capture Antibody (lyophilized) 2 vials
    Mouse M-CSF Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
  • Cellular localization

    Cell membrane; Single-pass membrane protein
  • Alternative names

    • Colony stimulating factor 1 (macrophage)
    • CSF-1
    • CSF1
    • Lanimostim
    • Macrophage colony-stimulating factor 1
    • MCSF
    • Processed macrophage colony-stimulating factor 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab199084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between mouse M-CSF SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of mouse serum, plasma (citrate) and stimulated L929 cell extracts. The 1X dilution for mouse serum was 6.25% matrix, and the 1X dilution for plasma was 50.0% matrix. The 1X dilution for the L929 extract was 500 µg/mL. The interpolated M-CSF concentrations multiplied by the total dilution factor are shown for each matrix (mean +/- SD, n = 2).

  • The 1X dilution for stimulated L929 supernatant was 12.5% matrix, and the 1X dilution for unstimulated L929 supernatant was 25.0% matrix. The interpolated M-CSF concentrations multiplied by the total dilution factor are shown for each matrix (mean +/- SD, n = 2).

Protocols

References

This product has been referenced in:

  • Jin X  et al. Advanced Glycation End Products Enhance Murine Monocyte Proliferation in Bone Marrow and Prime Them into an Inflammatory Phenotype through MAPK Signaling. J Diabetes Res 2018:2527406 (2018). Read more (PubMed: 29765986) »
See 1 Publication for this product

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