Overview

  • Product name

    Mouse MASPIN ELISA Kit
    See all MASPIN kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Skin extract 8 6.6%
  • Sample type

    Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    18.2 pg/ml
  • Range

    46.88 pg/ml - 3000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts 98 % - %

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    MASPIN in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of MASPIN protein in mouse tissue extract.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    MASPIN, also known as Serpin B5, is a serine protease inhibitor which plays a role in suppressing tumor metastasis in breast cancers. One of the known primary functions of MASPIN is the inhibition of growth factor -dependent cell migration. Current evidence suggests that dysregulation of this protein occurs in many types of cancer, however regulation is context-dependent, suggesting that both up and down-regulation of this gene is detrimental. MASPIN is currently under investigation as both a clinical marker for breast cancer diagnosis and prognosis as well as a target for cancer therapy.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse MASPIN Capture Antibody 1 x 600µl
    10X Mouse MASPIN Detector Antibody 1 x 600µl
    Mouse MASPIN Lyophilized Recombinant Protein 2 vials
    Antibody Diluent 4BR 1 x 6ml
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    50X Cell Extraction Enhancer Solution 1 x 1ml
    TMB Development Solution 1 x 12ml
    Stop Solution 1 x 12ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Plate Seals 1 unit
  • Research areas

  • Function

    Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • Tissue specificity

    Normal mammary epithelial cells.
  • Sequence similarities

    Belongs to the serpin family. Ov-serpin subfamily.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt
  • Alternative names

    • Maspin
    • Ovalbumin
    • Peptidase inhibitor 5
    • PI-5
    • PI5
    • Protease inhibitor 5
    • protease inhibitor 5 (maspin)
    • Serine (or cysteine) peptidase inhibitor, clade B, member 5
    • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5
    • Serpin B5
    • serpin family B member 5
    • Serpin peptidase inhibitor
    • Serpin peptidase inhibitor, clade B (ovalbumin), member 5
    • Serpinb5
    • SPB5_HUMAN
    • Spi5
    • Spi7
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab238270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of MASPIN were measured in duplicate and interpolated from the MASPIN standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MASPIN concentration was determined to be 1,060.3 pg/mL in mouse skin extract and 2,199.6 pg/mL in mouse small intestine extract.

Protocols

References

ab238270 has not yet been referenced specifically in any publications.

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