Overview

  • Product name

  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Mouse serum 5 7.3%
    Inter-assay
    Sample n Mean SD CV%
    Mouse serum 4 14%
  • Sample type

    Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    8.5 pg/ml
  • Range

    39.063 pg/ml - 2500 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 79.5 75% - 86.8%
    Cell culture media 99.7 95.4% - 103.3%
    Heparin Plasma 96.6 88.8% - 101.2%
    EDTA Plasma 94.9 74.1% - 102.3%
    Citrate Plasma 102.7 90.8% - 117.3%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s Mouse MBL in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of MBL protein in mouse serum, plasma, cell culture supernatant, and cell and tissue extract samples.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

     

    Sensitivity:

    Samples diluted in Sample Diluent NS - 11.2 pg/mL

    Samples diluted in 1X Cell Extraction Buffer PTR - 8.5 pg/mL

  • Notes

    MBL is a secreted calcium-dependent lectin involved in innate immune defense. MBL binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. MBL binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse MBL Capture Antibody 1 x 600µl
    10X Mouse MBL Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BR 1 x 6ml
    Mouse MBL Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 x 96 tests
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Function

    Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
  • Tissue specificity

    Plasma protein produced mainly in the liver.
  • Sequence similarities

    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Domain

    The coiled-coil domain mediates trimerization.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Collectin-1
    • Mannan-binding protein
    • Mannose-binding lectin
    • Mannose-binding protein C
    • MBL2
    • MBL2_HUMAN
    • MBP-C
    • MBP1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab208343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of MBL were measured in duplicates, interpolated from the MBL standard curves and corrected for sample dilution. 1X diluted samples are pre-diluted as follows: serum 1:83, plasma (citrate) 1:83, plasma (EDTA) 1:167, plasma (heparin) 1:167. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MBL concentration was determined to be 169.0 ng/mL in serum, 116.5 ng/mL in plasma (citrate), 179.8 ng/mL in plasma (EDTA) and 148.4 ng/mL in plasma (heparin).

  • Mouse liver cells were cultured for 5 days in a medium and supernatant sample was prepared. The concentrations of MBL were measured in duplicates, interpolated from the MBL standard curves and corrected for sample dilution. 1X diluted samples are pre-diluted as follows: liver supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MBL concentration was determined to be 4.3 ng/mL in liver supernatant.

  • The concentrations of MBL were measured in duplicate and interpolated from the MBL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MBL concentration was determined to be 1.56 ng/mL in liver extract sample.

  • The concentrations of MBL were measured in three different dilutions in duplicate and interpolated from the MBL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of MBL per mg of extract (mean +/- SD, n=3).

Protocols

References

ab208343 has not yet been referenced specifically in any publications.

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