Overview

  • Product name

    Mouse MCP-5 ELISA Kit
    See all MCP5 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 3.1%
    Inter-assay
    Sample n Mean SD CV%
    serum 3 3.8%
  • Sample type

    Cell culture supernatant, Serum, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    6 pg/ml
  • Range

    27.56 pg/ml - 1700 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 111 106% - 114%
    Serum 116 113% - 119%
    Cit plasma 103 100% - 106%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    MCP-5 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of MCP-5 protein in mouse serum, plasma (citrate), and tissue supernatants.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB Development Solution is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Monocyte chemotactic protein 5 (MCP-5), also known as C-C motif chemokine 12 (CCL12), is a chemotactic factor encoded by the gene CCL12. MCP-5 is inducibility expressed by interferon gamma or lipopolysaccharides (LPS) as a 104- amino acid protein, containing a 22- amino acid signal peptide and 82- amino acid chain, which can then form homodimers. MCP-5 attracts eosinophils, lymphocytes, and monocytes. MCP-5 is thought to coordinate cell movements during allergic reactions or as a response to pathogens.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse MCP-5 Capture Antibody 1 x 600µl
    10X Mouse MCP-5 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse MCP-5 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
  • Tissue specificity

    Predominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C-C motif chemokine 12
    • Ccl12
    • CCL12_MOUSE
    • Macrophage/monocyte Chemotactic Protein
    • MCP-1-related chemokine
    • MCP-5
    • Monocyte chemoattractant protein 5
    • Monocyte chemotactic protein 5
    • OTTMUSP00000000792
    • Scya12
    • Small inducible cytokine A12
    • Small-inducible cytokine A12
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab246547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • The MCP-5 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of MCP-5 were measured in duplicates and interpolated from the MCP-5 standard curves. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, and lung supernatant 37.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

     

Protocols

References

ab246547 has not yet been referenced specifically in any publications.

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