Overview

  • Product name

    Mouse MCP2 Matched Antibody Pair Kit
    See all MCP2 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    3.9 pg/ml - 250 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse MCP2. Both capture and detector antibodies are rabbit monoclonal antibodies.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Additional protocol information and tip on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab203366) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Buffer information:
    The capture antibody is glycerol free.
    The detector antibody contains glycerol.

  • Tested applications

    Suitable for: ELISA, Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Mouse MCP2 Capture Antibody 1 x 100µg 1 x 20µg
    Mouse MCP2 Detector Antibody 1 x 25µg 1 x 5µg
    Mouse MCP2 Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.
  • Tissue specificity

    Highest expression found in the small intestine and peripheral blood cells. Intermediate levels seen in the heart, placenta, lung, skeletal muscle, thymus, colon, ovary, spinal cord and pancreas. Low levels seen in the brain, liver, spleen and prostate.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Ccl8
    • CCL8_HUMAN
    • HC14
    • MCP-2
    • MCP-2(6-76)
    • Monocyte chemoattractant protein 2
    • Monocyte chemotactic protein 2
    • SCYA10
    • SCYA8
    • Small-inducible cytokine A8
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab213464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab213464 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213464.
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