Overview

  • Product name

    Mouse MCP3 ELISA Kit (CCL7)
    See all MCP3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 8 3.24%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 2.58%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.53 pg/ml
  • Range

    6.25 pg/ml - 400 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 95 91% - 99%
    Cell culture media 89 89% - 91%
    Heparin Plasma 85 80% - 87%
    EDTA Plasma 93 88% - 96%
    Citrate Plasma 98 94% - 102%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat
    Does not react with: Rabbit, Goat, Guinea pig, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s MCP3 (CCL7) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of MCP3 protein in mouse serum, plasmas, and cell culture supernatants.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    MCP3, also known as CCL7, belongs to the C-C chemokine family. MCP3 attracts monocytes, eosinophils, NK cells, T-lymphocytes, but not neutrophils. The ability of MCP3 to interact with various immune cell types are explained by the molecule’s ability to interact with various chemokine receptors. The chemokines known to interact with MCP3 include CCR1, CCR2, and CCR3. MCP3 also augments monocyte anti-tumor activity, and has been implicated in various studies investigating cancer metastasis.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab205571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The 1X dilution was 25% for the mouse serum and all of the mouse plasmas. Interpolated data values (mean +/- SD, n = 2) are graphed.

  • A blank cell culture media control was also analyzed, and was negative. The 1X dilution was 3.13% for the stimulated and unstimulated Raw 264.7 supernatants, and 25.0% for the unstimulated D10.G4.1 supernatant. Interpolated data values (mean +/- SD, n = 2) are graphed.

Protocols

References

This product has been referenced in:

  • Young LR  et al. Epithelial-macrophage interactions determine pulmonary fibrosis susceptibility in Hermansky-Pudlak syndrome. JCI Insight 1:e88947 (2016). ELISA ; Mouse . Read more (PubMed: 27777976) »
See 1 Publication for this product

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