Overview

  • Product name

    Mouse MCP3 Matched Antibody Pair Kit
    See all MCP3 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    1.56 pg/ml - 100 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse MCP3. Both capture and detector antibodies are rabbit monoclonal antibodies.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification. 


    Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab205571) which uses the same antibody pair. 


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Mouse MCP3 Capture Antibody 1 x 100µg 1 x 20µg
    Mouse MCP3 Detector Antibody 1 x 25µg 1 x 5µg
    Mouse MCP3 Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    O-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C-C motif chemokine 7
    • Ccl7
    • CCL7_HUMAN
    • Chemokine CC motif ligand 7
    • FIC
    • MARC
    • MCP-3
    • Monocyte chemoattractant protein 3
    • Monocyte chemotactic protein 3
    • NC28
    • RP23-350G1.4
    • SCYA6
    • SCYA7
    • Small-inducible cytokine A7
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab210889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab210889 has not yet been referenced specifically in any publications.

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