Overview

  • Product name

    Mouse MDC Matched Antibody Pair Kit
    See all MDC kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    7.81 pg/ml - 500 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse MDC. Both capture and detector antibodies are rabbit monoclonal antibodies.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification. 


    Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab204525) which uses the same antibody pair. 


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Buffer information:
    The capture antibody is glycerol free.
    The detector antibody contains glycerol.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Mouse MDC Capture Antibody 1 x 100µg 1 x 20µg
    Mouse MDC Detector Antibody 1 x 25µg 1 x 5µg
    Mouse MDC Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.
  • Tissue specificity

    Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Post-translational
    modifications

    The N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • A 152E5.1
    • ABCD 1
    • ABCD1
    • C C motif chemokine 22
    • CC chemokine STCP 1
    • CC chemokine STCP-1
    • ccl 22
    • Ccl22
    • CCL22_HUMAN
    • Chemokine (C C motif) ligand 22
    • DC/B CK
    • DCBCK
    • Macrophage-derived chemokine
    • MDC
    • MDC(1-69)
    • MDC(7-69)
    • MGC34554
    • SCYA22
    • Small inducible cytokine subfamily A (Cys Cys) member 22
    • Small inducible cytokine subfamily A, member 22
    • Small-inducible cytokine A22
    • STCP 1
    • STCP1
    • Stimulated T cell chemotactic protein 1
    • Stimulated T-cell chemotactic protein 1
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab211760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab211760 has not yet been referenced specifically in any publications.

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