Key features and details
- One-wash 90 minute protocol
- Sensitivity: 14 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse Mesothelin ELISA Kit
Intra-assay Sample n Mean SD CV% Overall 5 8.3% Inter-assay Sample n Mean SD CV% Overall 3 9.8%
Sample typeCell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range125 pg/ml - 8000 pg/ml
Sample specific recovery Sample type Average % Range Serum 119.6 115.6% - 122.8% Cell culture media 88.5 85.6% - 90% EDTA Plasma 116.3 114.4% - 118.8% Cit plasma 112.1 108.2% - 114.6%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Mouse
Does not react with: Pig
Mouse Mesothelin ELISA Kit (ab204528) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mesothelin protein in cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Mesothelin with 14 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Mesothelin is expressed as a much larger, 70 kDa precursor, which is cleaved into two functional proteins, Megakaryocyte potentiating factor and Mesothelin. Mesothelin is a glycosylated cell-surface protein, anchored to the plasma membrane via its GPI anchor. Membrane-anchored forms of Mesothelin may play a role in cellular adhesion.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Mouse Mesothelin Capture Antibody 1 x 600µl 10X Mouse Mesothelin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Mouse Mesothelin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
Cellular localizationSecreted and Cell membrane. Golgi apparatus.
- CAK1 antigen
- Megakaryocyte potentiating factor
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of Mesothelin were measured in duplicates, interpolated from the Mesothelin standard curve and corrected for sample dilution. Note that 1X Diluted samples were pre-diluted to 50%. The interpolated, dilution factor-corrected values are plotted in pg of Mesothelin per mL of neat sample (mean +/- SD, n=2).
The concentrations of Mesothelin were measured in duplicates, interpolated from the Mesothelin standard curve and corrected for sample dilution. Note that 1X Diluted C2C12 cell extract samples were pre-diluted to 125 µg/mL. Note that 1X Diluted NIH/3T3 cell extract samples were pre-diluted to 250 µg/mL. The interpolated, dilution factor-corrected values are plotted in pg of Mesothelin per mg of extract (mean +/- SD, n=2).
ab204528 has not yet been referenced specifically in any publications.