Overview

  • Product name

    Mouse Milk Fat Globule 1 Matched Antibody Pair Kit
    See all Milk Fat Globule 1 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    48.9 pg/ml - 6000 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant Milk Fat Globule 1.


    Both capture and detector antibodies are rabbit monoclonal antibodies.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website.


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab208981) which uses the same antibody pair.


    Buffer information: The capture antibody is glycerol free. The detector antibody contains glycerol.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Mouse Milk Fat Globule 1 Capture Antibody 1 x 100µg 1 x 20µg
    Mouse Milk Fat Globule 1 Detector Antibody 1 x 25µg 1 x 5µg
    Mouse Milk Fat Globule 1 Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
    Medin is the main constituent of aortic medial amyloid.
  • Tissue specificity

    Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
  • Sequence similarities

    Contains 1 EGF-like domain.
    Contains 2 F5/8 type C domains.
  • Post-translational
    modifications

    Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
  • Cellular localization

    Membrane. Secreted.
  • Information by UniProt
  • Alternative names

    • BA46
    • Breast epithelial antigen BA46
    • EDIL1
    • HMFG
    • hP47
    • HsT19888
    • Lactadherin
    • Medin
    • MFG-E8
    • MFGE8
    • MFGM
    • MFGM_HUMAN
    • Milk fat globule EGF factor 8
    • Milk fat globule EGF factor 8 protein
    • Milk fat globule-EGF factor 8
    • O acetyl disialoganglioside synthase
    • OAcGD3S
    • SED1
    • SPAG10
    • Sperm associated antigen 10
    • Sperm surface protein hP47
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab216785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

Protocols

References

ab216785 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab216785.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up