Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.95 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse MIP1a ELISA Kit (CCL3 )
See all MIP-1 alpha/CCL3 kits
Intra-assay Sample n Mean SD CV% Overall 5 1.44% Inter-assay Sample n Mean SD CV% Overall 3 1.97%
Sample typeCell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma
Assay typeSandwich (quantitative)
Range15.6 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Urine 81 75% - 85% Serum 81 80% - 83% Cell culture media 87 91% - 93% Cit plasma 82 78% - 86% serum free media 95 93% - 96%
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Mouse
Does not react with: Goat, Cow, Pig
Mouse MIP1a ELISA Kit (CCL3 ) (ab200017) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP1a (CCL3) protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MIP1a (CCL3) with 1.95 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Macrophage Inflammatory Protein 1 alpha (MIP1a, also known as C-C motif chemokine 3 or CCL3) is a secreted monokine with inflammatory and chemokinetic properties. Mature MIP1a is 68 amino acids after cleavage of a 23 amino acid signal peptide. MIP1a can be induced in a variety of cell types and for immune and inflammatory response. In humans, MIP1a is a major HIV-suppressive factor produced by CD8+ T cells. The standard protein in this kit is mature mouse MIP1a.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Mouse MIP1a Capture Antibody 1 x 600µl 10X Mouse MIP1a Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Mouse MIP1a Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
FunctionMonokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Sequence similaritiesBelongs to the intercrine beta (chemokine CC) family.
modificationsN-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
- Information by UniProt
- C C motif chemokine 3
- CCL 3
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
RAW264.7 cells were grown in the absence (unstimulated) or presence of LPS (stimulated) for 2 days. MIP1a was measured in 2-fold diluted cell culture supernatants of unstimulated and LPS stimulated RAW264.7 and cell culture media. Measured values were interpolated from the MIP1a standard curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 20 ng/mL unstimulated, 118 ng/mL stimulated, and undetectable in media.
The concentrations of MIP1a were measured in duplicate and interpolated from the MIP1a standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP1a concentration was determined to be 118 µg/mL in RAW264.7 LPS Stimulated supernatant.
ab200017 has been referenced in 3 publications.
- Clua P et al. The Role of Alveolar Macrophages in the Improved Protection against Respiratory Syncytial Virus and Pneumococcal Superinfection Induced by the Peptidoglycan of Lactobacillus rhamnosus CRL1505. Cells 9:N/A (2020). PubMed: 32660087
- Monga S et al. The Efficacy of the Novel TSPO Ligands 2-Cl-MGV-1 and 2,4-Di-Cl-MGV-1 Compared to the Classical TSPO Ligand PK 11195 to Counteract the Release of Chemokines from LPS-Stimulated BV-2 Microglial Cells. Biology (Basel) 9:N/A (2020). PubMed: 32938018
- Lee JW et al. The HIV co-receptor CCR5 regulates osteoclast function. Nat Commun 8:2226 (2017). PubMed: 29263385