• Product name

    Mouse MIP2 ELISA Kit (CXCL2)
    See all CXCL2 kits
  • Detection method

  • Sample type

    Cell culture supernatant, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 8 pg/ml
  • Range

    4.1 pg/ml - 1000 pg/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.58 86% - 104%
    Plasma 87.94 82% - 98%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s MIP2 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP2 in plasma and cell culture supernatants. (Mouse MIP2 concentration is pretty low in normal plasma, it may not be detected in this assay).

    This assay employs an antibody specific for mouse MIP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    We have not been able to detect endogenous Mouse MIP2 in normal serum with ab100727, only in serum spiked with Mouse MIP2.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform



  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    700X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-mouse MIP2 (lyophilized) 2 vials
    MIP2 Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse MIP2 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational

    The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells.
  • Cellular localization

  • Information by UniProt
  • Alternative names

    • C-X-C motif chemokine 2
    • Chemokine (C-X-C motif) ligand 2
    • CINC-2a
    • Cxcl2
    • Gro beta
    • Gro-beta
    • GRO-beta(5-73)
    • GRO-beta-T
    • GRO2
    • GRO2 oncogene
    • GROb
    • Growth-regulated protein beta
    • Hematopoietic synergistic factor
    • HSF
    • Macrophage inflammatory protein 2-alpha
    • Melanoma growth stimulatory activity beta
    • MGSA beta
    • MGSA-b
    • MIP 2
    • MIP 2a
    • MIP2
    • MIP2-alpha
    • MIP2A
    • SB-251353
    • SCYB2
    see all
  • Database links


Our Abpromise guarantee covers the use of ab100727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab100727

  • Representative standard curve using ab100727



ab100727 has not yet been referenced specifically in any publications.

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