Overview

  • Product name

    Mouse MIP2 ELISA Kit (CXCL2)
    See all CXCL2 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    overall 8 1.64%
    Inter-assay
    Sample n Mean SD CV%
    overall 3 4.87%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    0.87 pg/ml
  • Range

    3.13 pg/ml - 200 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 108 103% - 113%
    Cell culture media 102 98% - 107%
    Heparin Plasma 104 101% - 109%
    EDTA Plasma 115 113% - 116%
    Citrate Plasma 91 87% - 97%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Rat, Rabbit, Hamster, Dog, Human
  • Product overview

    Abcam’s MIP2 (CXCL2) Mouse in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of MIP2 protein in mouse serum, plasma, and cell culture supernatants.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

    Sensitivity:

    Samples diluted in Sample Diluent NS – 0.87 pg/mL

    Samples diluted in Sample Diluent 50BP – 1.62 pg/mL

    Samples diluted in Sample Diluent 25BP – 1.03 pg/mL

  • Notes

    Mouse Macrophage Inflammatory Protein-2 (MIP2), also known as C-X-C motif chemokine 2 (CXCL2), is a small cytokine belonging to the CXC chemokine family. MIP2 was originally identified as a heparin-binding protein, and has been shown to exhibit potent neutrophil chemotactic activity.

    Mouse MIP2 c-DNA encodes a 100 amino acid residue precursor protein. The amino-terminal 27 amino acid residues are cleaved from this precursor to generate the mature mouse MIP2. Mouse MIP2 is 63% identical to Mouse KC (another mouse alpha chemokine), and mouse MIP2 is 60% identical to human GROβ and GROγ. Based on these protein sequence similarities, it is likely that mouse MIP2 and KC are homologs of the human GROα, β, and γ chemokines. However, since chemokines with protein sequence homology to human IL-8 have not been identified in mice, it has been suggested that the mouse MIP2 and KC are functional homologs of human IL-8 in mice. A putative mouse homolog of the human IL-8 receptor beta (IL-8 Rβ) has also been cloned. This receptor shows 71% identity to human IL-8 Rβ and 68% identity to human IL-8 Rα. Both mouse MIP2 and KC bind mouse IL-8 Rβ with high affinity.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse MIP2 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Mouse MIP2 Capture Antibody (Lyophilized) 1 vial
    Mouse MIP2 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 50BP 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Function

    Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    The N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • C-X-C motif chemokine 2
    • Chemokine (C-X-C motif) ligand 2
    • CINC-2a
    • Cxcl2
    • CXCL2_HUMAN
    • Gro beta
    • Gro-beta
    • GRO-beta(5-73)
    • GRO-beta-T
    • GRO2
    • GRO2 oncogene
    • GROb
    • Growth-regulated protein beta
    • Hematopoietic synergistic factor
    • HSF
    • Macrophage inflammatory protein 2-alpha
    • Melanoma growth stimulatory activity beta
    • MGSA beta
    • MGSA-b
    • MIP 2
    • MIP 2a
    • MIP2
    • MIP2-alpha
    • MIP2A
    • SB-251353
    • SCYB2
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab204517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between mouse MIP2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit show comparable sensitivity.

  • Recombinant mouse MIP2 was spiked into 100% serum and diluted in a 2-fold dilution series in Sample Diluent 50BP. Recombinant mouse MIP2 was spiked into 10% cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse MIP2 were measured in duplicate and interpolated from the mouse MIP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

  • Recombinant mouse MIP2 was spiked into 100% citrate plasma and 50% EDTA plasma and diluted in a 2-fold dilution series in Sample Diluent 25BP. Recombinant mouse MIP2 was spiked into 100% heparin plasma and diluted in a 2-fold dilution series in Sample Diluent 50BP. The concentrations of mouse MIP2 were measured in duplicate and interpolated from the mouse MIP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

  • Native MIP2 was measured in duplicate in 100% serum and 100% heparin plasma and concentrations interpolated from a standard curve diluted in Sample Diluent 50BP. Native MIP2 was measured in duplicate in 100% Citrate plasma and 50% EDTA plasma and concentrations interpolated from a standard curve diluted in Sample Diluent 25BP. The interpolated dilution factor corrected values are graphed (mean +/- SD).

  • J774A.1 cells were cultured in HGDMEM with 10% fetal calf serum, and 100 µg/mL of Kanamycin. During the exponential growth phase, J774A.1 cells were treated for 72 hours in the presence and absence of 1.5% PHA and 10 ng/mL of PMA. RAW 264.7 cells were cultured in HGDMEM with 10% fetal calf serum, 2 mM L-glutamine and 100 µg/mL Kanamycin. During the exponential growth phase, RAW264.7 cells were starved for 24 hours and treated in the presence and absence of 5 µg/mL of LPS (Batch #1) or 1% PHA (Batch #2).The concentrations of mouse MIP2 were interpolated from a standard curve diluted in Sample Diluent NS and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

  • Background-subtracted data values (mean +/- SD) are graphed.

Protocols

References

ab204517 has not yet been referenced specifically in any publications.

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