Mouse MIP3 alpha ELISA Kit (CCL20) (ab100728)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 2.06 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
-
Product name
Mouse MIP3 alpha ELISA Kit (CCL20)
See all Macrophage Inflammatory Protein 3 alpha kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 3 pg/ml -
Range
2.06 pg/ml - 1500 pg/ml -
Recovery
90 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 80.52 72% - 91% Serum 83.74 74% - 93% Plasma 108.2 84% - 143% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s MIP3 alpha Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MIP3 alpha in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MIP3 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP3 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MIP3 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP3 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 unit 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-mouse MIP3 alpha 2 vials MIP3 alpha Microplate (12 x 8 wells) 1 unit Recombinant Mouse MIP3 alpha Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. -
Tissue specificity
Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsC-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Beta chemokine exodus 1
- Beta-chemokine exodus-1
- C C motif chemokine ligand 20
see all -
Database links
- Entrez Gene: 20297 Mouse
- SwissProt: O89093 Mouse
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab100728 has been referenced in 1 publication.
- Sakamoto K et al. Disruption of the endopeptidase ADAM10-Notch signaling axis leads to skin dysbiosis and innate lymphoid cell-mediated hair follicle destruction. Immunity 54:2321-2337.e10 (2021). PubMed: 34582748