Overview

  • Product name

    Mouse MMP-12 ELISA Kit
    See all MMP12 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 337pg/ml 18.2 5.4%
    2 16 1472pg/ml 92.74 6.3%
    3 16 2522pg/ml 121.1 4.8%
    Inter-assay
    Sample n Mean SD CV%
    1 24 426pg/ml 27.69 6.5%
    2 24 1538pg/ml 109.2 7.1%
    3 24 2768pg/ml 157.8 5.7%
  • Sample type

    Cell culture supernatant, Serum, Heparin Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse MMP-12 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213878) is designed for the quantitative detection of Mouse MMP-12 in cell culture supernatants, serum and plasma (heparin). 


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for MMP-12 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: A18-C462) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse MMP-12 amount of sample captured in plate.

  • Notes

    Matrix metalloproteinase-12 (MMP12), also known as MME or ME, is an enzyme that in humans is encoded by the MMP12 gene. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.2. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. This gene may be involved in tissue injury and remodeling.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Mouse MMP-12 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Mouse MMP-12 antibody 1 x 130µl
    Lyophilized recombinant Mouse MMP-12 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
  • Tissue specificity

    Found in alveolar macrophages but not in peripheral blood monocytes.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names

    • EC 3.4.24.65
    • HME
    • Macrophage elastase
    • Macrophage metalloelastase
    • Macrophage metaloelastase
    • Matrix metallopeptidase 12 (macrophage elastase)
    • Matrix metalloprotease 12
    • Matrix metalloproteinase-12
    • ME
    • MGC138506
    • MME
    • MMP 12
    • MMP-12
    • Mmp12
    • MMP12_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse MMP-12 ELISA Kit (ab213878) Standard Curve

Protocols

References

ab213878 has not yet been referenced specifically in any publications.

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