Overview

  • Product name

    Mouse MMP3 ELISA Kit
    See all MMP3 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 3.2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 4.8%
  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, Cit plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    4.94 pg/ml
  • Range

    18.8 pg/ml - 1200 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 103 99% - 109%
    Serum 112 101% - 126%
    Hep Plasma 103 97% - 110%
    Cit plasma 105 97% - 115%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Pig
  • Product overview

    Abcam’s MMP3 in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of MMP3 protein in mouse serum, plasma and cell culture supernatant.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Matrix metalloproteinase-3 (MMP3) is a member of the matrix metalloproteinase family, which are involved in breakdown of the extracellular matrix. MMP3 is secreted in a proenzyme form from a variety of cells, including macrophages, osteoblasts, keratinocytes, chondrocytes, fibroblasts and vascular smooth muscle cells. It degrades collagen types II, III, IV, IX and X, along with proteoglycans, elastin, laminin and fibronectin. In addition to normal physiological processes like development, reproduction and wound healing, MMP3 is implicated in humans in the progression of atherosclerosis and tumor initiation.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab203363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Standard curve comparison between mouse MMP3 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 10-fold increase in sensitivity.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of MMP3 were measured in duplicate and interpolated from the MMP3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Protocols

References

ab203363 has not yet been referenced specifically in any publications.

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