Key features and details
- Mouse monoclonal [2A 8F4] Anti-Rat IgG2a heavy chain (Alexa Fluor® 647)
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Host species: Mouse
- Isotype: IgG1
- Suitable for: IHC-Fr, Flow Cyt
Product nameMouse monoclonal [2A 8F4] Anti-Rat IgG2a heavy chain (Alexa Fluor® 647)
See all IgG2a secondary antibodies
SpecificityThis antibody is specific to Rat IgG2a heavy chain
Tested applicationsSuitable for: IHC-Fr, Flow Cytmore details
The details of the immunogen for this antibody are not available.
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: 23% Glycerol, PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Clone number2A 8F4
Light chain typekappa
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
Our Abpromise guarantee covers the use of ab172333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||1/2000 - 1/3000.|
Overlay histogram showing subclass specificity of ab172333. HeLa cells were fixed with 80% methanol (-20ºC, 5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% serum / 0.3M glycine to block non-specific protein-protein interactions followed by the primary antibody (1μg/1x106) as indicated: ab16881 (Rat IgG1), ab90458 (Rat IgG2a), ab81461 (Rat IgG2b), ab50610 (Rat IgGc) for 30 min at 22ºC. The secondary antibody (ab172333 - Mouse monoclonal (2A 8F4) Anti-Rat IgG2a, heavy chain - Alexa Fluor® 647) was used at 1/3000 dilution for 30 min at 22ºC. Unlabelled (blue line) and secondary only (gold line) samples were also used as negative controls. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635nm) and 675/30 bandpass filter.
Emission spectra of Alexa Fluor® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab172333 has been referenced in 2 publications.
- Nguyen TT et al. The IgM receptor FcµR limits tonic BCR signaling by regulating expression of the IgM BCR. Nat Immunol 18:321-333 (2017). PubMed: 28135254
- Qi S et al. Long-term intravital imaging of the multicolor-coded tumor microenvironment during combination immunotherapy. Elife 5:N/A (2016). IHC-P . PubMed: 27855783