an abreview for ab99702 - Mouse monoclonal 2A9 Anti-Rabbit IgG heavy chain (HRP)

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Western blot
We used rabbit polyconial iNOS to immunoprecipitate HMGB1 and detect the association by immunoblotting of HMGB1 (ab18265). Due to the size of HMGB1, the IgG light chain masked the HMGB1 signal in iNOS immunoprecipitated samples. To avoid the effects from IgG light chain, Mouse monoclonal 2A9 Anti-Rabbit IgG heavy chain (HRP) (ab99702) is chosen to replace the goat anti-rabbit IgG (H&L) HRP-conjugated secondary antibody from Cell Signaling.

As indicated in the image, Mouse monoclonal 2A9 Anti-Rabbit IgG heavy chain (HRP) (ab99702) is shown in the left panel while the goat anti-rabbit IgG (H&L) HRP-conjugated secondary antibody from Cell Signaling is shown in the right panel.

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Submitted Sep 02 2014

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