Mouse monoclonal [4E3] Anti-Human IgG1 gamma chain (Biotin) (ab99775)

Overview

  • Product name

    Mouse monoclonal [4E3] Anti-Human IgG1 gamma chain (Biotin)
    See all IgG1 secondary antibodies
  • Host species

    Mouse
  • Target species

    Human
  • Specificity

    ab99775 reacts with the hinge region of the heavy chain of Human IgG1. May also react with IgG from other species.
  • Tested applications

    Suitable for: ELISA, ICC/IFmore details
  • Immunogen

    Other Immunogen Type corresponding to IgG1.

  • Conjugation

    Biotin

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    4E3
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/2000.
ICC/IF Use at an assay dependent dilution.

References

This product has been referenced in:

  • Vidal M  et al. Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens. J Immunol Methods N/A:N/A (2018). Read more (PubMed: 29397157) »
  • Ubillos I  et al. Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1-4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies. Malar J 17:219 (2018). Read more (PubMed: 29859096) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

ab1927 does not cross react with mouse IgGs so it could also be a option to try.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

Antibody ab50473 does not react with mouse IgGs. This might be suitable as per your requirements.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

It seems product ab1927 and ab50473, could be suitable however in order to confirm it I am now waiting, species cross reactivity data from lab. I will get back to you with more updates soon.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up