Mouse monoclonal [4E3] Anti-Human IgG1 gamma chain (Biotin) (ab99775)

Submit an Abreview Q&A (3)Specific References (3)

Overview

  • Product name
    Mouse monoclonal [4E3] Anti-Human IgG1 gamma chain (Biotin)
    See all IgG1 secondary antibodies
  • Host species
    Mouse
  • Target species
    Human
  • Specificity
    ab99775 reacts with the hinge region of the heavy chain of Human IgG1. May also react with IgG from other species.
  • Tested applications
    Suitable for: ELISA, ICC/IFmore details
  • Immunogen

    Other Immunogen Type corresponding to IgG1.

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    4E3
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/2000.
ICC/IF Use at an assay dependent dilution.

References

This product has been referenced in:
  • Vidal M  et al. Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens. J Immunol Methods N/A:N/A (2018). Read more (PubMed: 29397157) »
  • Ubillos I  et al. Optimization of incubation conditions of Plasmodium falciparum antibody multiplex assays to measure IgG, IgG1-4, IgM and IgE using standard and customized reference pools for sero-epidemiological and vaccine studies. Malar J 17:219 (2018). Read more (PubMed: 29859096) »

See all 3 Publications for this product

Publishing research using ab99775? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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3 reviews or Q&A

10
Votes: Highest First

I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
Does your company provid...

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Thank you for contacting us.

ab1927 does not cross react with mouse IgGs so it could also be a option to try.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.<...

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I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
Does your company provid...

Read More

Thank you for contacting us.

Antibody ab50473 does not react with mouse IgGs. This might be suitable as per your requirements.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice...

Read More

I am looking for a secondary antibody, which detects human IgG1 specifically without cross reactivity towards murine Fc. I would like to use such antibody in a FACS staining and the labeling of the antibody does not matter.
Does your company provid...

Read More

Thank you for contacting us.

It seems product ab1927 and ab50473, could be suitable however in order to confirm it I am now waiting, species cross reactivity data from lab. I will get back to you with more updates soon.

I hope this in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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