Mouse monoclonal [4E3] Anti-Human IgG1 hinge heavy chain (Alkaline Phosphatase) (ab99773)
Key features and details
- Mouse monoclonal [4E3] Anti-Human IgG1 hinge heavy chain (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Mouse
- Isotype: IgG1
- Suitable for: ELISA, Flow Cyt
Related conjugates and formulations
Overview
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Product name
Mouse monoclonal [4E3] Anti-Human IgG1 hinge heavy chain (Alkaline Phosphatase)
See all IgG1 secondary antibodies -
Host species
Mouse -
Target species
Human -
Specificity
ab99773 reacts with the hinge region of the heavy chain of Human IgG1 as demonstrated by ELISA. May also react with IgG from other species. -
Tested applications
Suitable for: ELISA, Flow Cytmore details -
Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.00
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.0095% Magnesium chloride, 0.605% Tris -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
4E3 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- Mouse monoclonal [4E3] Anti-Human IgG1 hinge heavy chain (ab99771)
- Mouse monoclonal [4E3] anti- Human IgG1 - hinge heavy chain (FITC) (ab99772)
- Mouse monoclonal [4E3] Anti-Human IgG1 hinge heavy chain (HRP) (ab99774)
- Mouse monoclonal [4E3] Anti-Human IgG1 gamma chain (Biotin) (ab99775)
- Mouse monoclonal [4E3] Anti-Human IgG1 hinge heavy chain (PE) (ab99776)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/500 - 1/1000.
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Flow Cyt |
Use at an assay dependent dilution.
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Notes |
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ELISA
1/500 - 1/1000. |
Flow Cyt
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab99773 has been referenced in 2 publications.
- Cao Y et al. Effect of TNF-a on the proliferation and osteogenesis of human periodontal mesenchymal stem cells. Exp Ther Med 21:434 (2021). PubMed: 33747173
- Whary MT et al. Intestinal helminthiasis in Colombian children promotes a Th2 response to Helicobacter pylori: possible implications for gastric carcinogenesis. Cancer Epidemiol Biomarkers Prev 14:1464-9 (2005). PubMed: 15941957