Mouse monoclonal [4E3] anti- Human IgG1 - hinge heavy chain (FITC) (ab99772)

Overview

  • Product name

    Mouse monoclonal [4E3] anti- Human IgG1 - hinge heavy chain (FITC)
    See all IgG1 secondary antibodies
  • Host species

    Mouse
  • Target species

    Human
  • Specificity

    ab99772 reacts with the hinge region of the heavy chain of Human IgG1 as demonstrated by ELISA. May also react with IgG from other species.
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, ELISAmore details
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm

Properties

Applications

Our Abpromise guarantee covers the use of ab99772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use 1µg for 106 cells.
Flow Cyt Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.

References

This product has been referenced in:

  • Manso C  et al. Anti-Neurofascin-155 IgG4 antibodies prevent paranodal complex formation in vivo. J Clin Invest 130:2222-2236 (2019). Read more (PubMed: 30869655) »
  • Wang JH  et al. Upregulation of microRNA-143 reverses drug resistance in human breast cancer cells via inhibition of cytokine-induced apoptosis inhibitor 1. Oncol Lett 13:4695-4700 (2017). WB . Read more (PubMed: 28588724) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

ab1927 does not cross react with mouse IgGs so it could also be a option to try.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

Antibody ab50473 does not react with mouse IgGs. This might be suitable as per your requirements.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us.

It seems product ab1927 and ab50473, could be suitable however in order to confirm it I am now waiting, species cross reactivity data from lab. I will get back to you with more updates soon.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

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