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  1. Link

    mouse-monoclonal-7c2-chicken-igy-hl-ab8922.pdf

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Secondary antibodies anti-Chicken IgY Unconjugated
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Mouse monoclonal [7C2] Anti-Chicken IgY H&L (ab8922)

  • Datasheet
Submit a review Q&A (1)

Product price, shipping and contact information

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Key features and details

  • Mouse monoclonal [7C2] Anti-Chicken IgY H&L
  • Host species: Mouse
  • Isotype: IgG1
  • Suitable for: IP, WB

Overview

  • Product name

    Mouse monoclonal [7C2] Anti-Chicken IgY H&L
    See all IgY secondary antibodies
  • Description

    Mouse monoclonal [7C2] Anti-Chicken IgY H&L
  • Host species

    Mouse
  • Target species

    Chicken
  • Tested applications

    Suitable for: IP, WBmore details

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    7C2
  • Isotype

    IgG1
  • Light chain type

    unknown
  • General notes

    The yolk of eggs laid by immunized chickens has been recognized as an excellent source of antibodies Specific antibodies produced in chickens offer several important advantages over producing antibodies in other mammals. Because a single egg contains as much antibody as an average bleed from a rabbit, this simple, non-invasive approach presents an appealing alternative to conventional antibody production methods. Purification of chicken egg yolk immunoglobulin Y (IgY), the 150 kDa IgG homolog, does not require animal bleeding. In addition, the eggs from immunized chickens provide a continual, daily source of antibody, and this convenient approach offers greater compatibility with animal protection regulations. Due to the phylogenetic distance between birds and mammals, there is greater potential of producing a higher percentage of specific antibody against mammalian antigens when using chickens. Highly conserved mammalian proteins sometimes fail to illicit a humoral response in animals, such as rabbits, that are traditionally used for generating antibodies. Non-specific binding and need for cross-species immunoabsorptions is eliminated since chicken IgY does not cross-react with mammalian IgG and does not bind bacterial or mammalian Fc receptors. There are well defined structural differences of IgY-type immunoglobulins and the IgG of mammals. That includes the molar mass of the heavy chains of the immunoglobulins. The IgY-type immunoglobulins are much less flexible than IgG. Also, the structures of the Fc part of the immunoglobulin isotypes IgY and IgG are different.

     

    The antibody can be used during various purification steps of IgY.

  • Research areas

    • Secondary antibodies
    • anti-Chicken
    • IgY
    • Unconjugated

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
Notes
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab8922? Please let us know so that we can cite the reference in this datasheet.

    ab8922 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Submit a review Submit a question

    Question

    We recently ordered and received 4 vials of mouse anti-chicken IgY (Catalog # ab8922). When the items were received they were accidentally stored at -25 to -18C instead of 4C as specified on the data sheet supplied with the antibodies. They have since been removed from the freezer and placed into the refrigerator. We were wondering whether you have any stability data for this material after a freeze thaw. We just wanted to know whether the antibodies were still ok to use or whether they could have been compromised. If you could please let us know your thoughts, we would greatly appreciate it.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 02 2012

    Answer

    Thank you for your enquiry.

    I do not have any stability data available for this antibody. However I think it should be fine and I certainly think it is worth trying in your experiment. If you do see any precipitate from the freeze/thaw, simply pellet this with a quick spin in the centrifuge as the precipitate may cause high background.

    I hope this is helpful. Please contact me if you have any further questions.

    Read More

    Abcam Scientific Support

    Answered on Mar 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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