Key features and details
- Mouse monoclonal [B3102E8] Anti-Human IgE Fc
- Host species: Mouse
- Isotype: IgG1
- Suitable for: ELISA, WB, Dot blot, IHC-Fr, ICC
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.20
Constituent: 100% Borate buffered saline
No preservatives or amine-containing buffer salts added.
Concentration information loading...
Light chain typekappa
This product has switched from ascites to tissue culture supernatant on 8th June 2020. Lot numbers higher than GR3258714 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Our Abpromise guarantee covers the use of ab99804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution.|
|Dot blot||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
ELISA plate was coated with Human IgE. Immunoglobuliun was detected with serially diluted Mouse monoclonal [B3102E8] Anti-Human IgE Fc (ab99804) conjugated to Biotin followed by Streptavidin-HRP and ABTS.
This product has switched from ascites to tissue culture supernatant on 8th June 2020. Lot numbers higher than GR3258714 are from tissue culture supernatant.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab99804 has not yet been referenced specifically in any publications.