Mouse monoclonal [B3102E8] Anti-Human IgE Fc (Biotin) (ab99807)
Key features and details
- Mouse monoclonal [B3102E8] Anti-Human IgE Fc (Biotin)
- Conjugation: Biotin
- Host species: Mouse
- Isotype: IgG1
- Suitable for: ELISA, WB, Dot blot, IHC-Fr, ICC
Related conjugates and formulations
Overview
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Product name
Mouse monoclonal [B3102E8] Anti-Human IgE Fc (Biotin)
See all IgE secondary antibodies -
Host species
Mouse -
Target species
Human -
Specificity
ab99807 Reacts with the Fc portion of the heavy chain of Human IgE -
Tested applications
Suitable for: ELISA, WB, Dot blot, IHC-Fr, ICCmore details -
Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
B3102E8 -
Isotype
IgG1 -
Light chain type
kappa -
General notes
This product has switched from ascites to tissue culture supernatant on 8th June 2020. Lot numbers higher than GR3323141 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/1000 - 1/2000.
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WB |
Use at an assay dependent dilution.
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Dot blot |
Use at an assay dependent dilution.
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IHC-Fr |
Use at an assay dependent dilution.
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ICC |
Use at an assay dependent dilution.
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Notes |
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ELISA
1/1000 - 1/2000. |
WB
Use at an assay dependent dilution. |
Dot blot
Use at an assay dependent dilution. |
IHC-Fr
Use at an assay dependent dilution. |
ICC
Use at an assay dependent dilution. |
Images
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ELISA plate was coated with Human IgE. Immunoglobuliun was detected with serially diluted Mouse monoclonal [B3102E8] Anti-Human IgE Fc (Biotin) (ab99807) followed by Streptavidin-HRP and ABTS.
This product has switched from ascites to tissue culture supernatant on 8th June 2020. Lot numbers higher than GR3258714 are from tissue culture supernatant.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab99807 has been referenced in 7 publications.
- Wróblewska B et al. The Immune System Response to 15-kDa Barley Protein: A Mouse Model Study. Nutrients 14:N/A (2022). PubMed: 36297055
- Jia H et al. Actin-binding protein anillin promotes the progression of hepatocellular carcinoma in vitro and in mice. Exp Ther Med 21:454 (2021). PubMed: 33747188
- Wu S et al. Cyclin B2 (CCNB2) Stimulates the Proliferation of Triple-Negative Breast Cancer (TNBC) Cells In Vitro and In Vivo. Dis Markers 2021:5511041 (2021). PubMed: 34354775
- Sánchez-Salguero E et al. Maternal IgA2 Recognizes Similar Fractions of Colostrum and Fecal Neonatal Microbiota. Front Immunol 12:712130 (2021). PubMed: 34804008
- Huang M et al. Identification of AK4 as a novel therapeutic target for serous ovarian cancer. Oncol Lett 20:346 (2020). PubMed: 33123257
- Yue FR et al. SMYD3 promotes colon adenocarcinoma (COAD) progression by mediating cell proliferation and apoptosis. Exp Ther Med 20:11 (2020). PubMed: 32934676
- Tan Y et al. Application of photonic crystal enhanced fluorescence to detection of low serum concentrations of human IgE antibodies specific for a purified cat allergen (Fel D1). Biosens Bioelectron 77:194-201 (2016). PubMed: 26406461