Overview

  • Product name

    Mouse monoclonal [H2] Anti-Human IgG Fc (HRP)
    See all IgG secondary antibodies
  • Host species

    Mouse
  • Target species

    Human
  • Specificity

    ab99765 reacts with the Fc portion of the heavy chain of all subclasses of Human IgG as demonstrated by ELISA; may also react with IgG from other species.
  • Tested applications

    Suitable for: ELISA, WB, Dot blot, ICC, IHC-Frmore details
  • Immunogen

    Other Immunogen Type corresponding to IgG.

  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: None
    Constituents: 50% PBS, 50% Glycerol, pH 7.4
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    Purified from ascites.
  • Clonality

    Monoclonal
  • Clone number

    H2
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/20000.
WB Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

Images

  • ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted ab99765.

References

This product has been referenced in:

  • Hecker M  et al. High-Density Peptide Microarray Analysis of IgG Autoantibody Reactivities in Serum and Cerebrospinal Fluid of Multiple Sclerosis Patients. Mol Cell Proteomics 15:1360-80 (2016). Read more (PubMed: 26831522) »
  • Xing M  et al. Gene therapy for colorectal cancer using adenovirus-mediated full-length antibody, cetuximab. Oncotarget 7:28262-72 (2016). Read more (PubMed: 27058423) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abreviews
Application
Western blot
This study was carried out to identify fungal proteins that are specific for IgG antibodies in sera from human patients with an specific mycosis. Proteins were blotted onto nitrocellulose membranes and membranes were incubated with sera from different patients and at different dilutions. However, when the membranes were incubated with mouse monoclonal H2 anti-human IgG Fc HRP (ab99765) very few bands and with very weak intensity were detected (the experiment was simultaneously done with a goat anti-human IgG-HRP from a different company).

Abcam response: We would like to thank the reviewer for submitting the AbReview and the feedback about the use of ab99765 in Western Blot. ab99765 is a monoclonal antibody which may be the reason the intensity of the signal is lower than the antibody it is compared with.

Carolina Firacative

Verified customer

Submitted Jan 31 2018

Answer


We recommend storing this antibody undiluted at 4 degrees for the short term and at -20 degrees after aliquots have been made. We do not recommend storing this antibody in a diluted form (1:10,000 to 1:20,000) at any temperature as the antibody is likely to be very unstable. The antibody should only be diluted immediately prior to use.

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Question
Answer

Thank you for contacting us.

Yes, this will recognize the Fc domain of all human IgG subclasses, including IgG2.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for calling Abcam earlier today.

I am sorry that the first replacement antibody we sent you did not prove to be successful and so as we discussed I am sending you a vial of ab99765. Your order number, for your records for this product is *-****.

If there is anything else I can help you with, please let me know.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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