Mouse monoclonal [hIgG1] Anti-Human IgG H&L preadsorbed (ab436)

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Overview

  • Product name
    Mouse monoclonal [hIgG1] Anti-Human IgG H&L preadsorbed
    See all IgG secondary antibodies
  • Description
    Mouse monoclonal [hIgG1] Anti-Human IgG H&L preadsorbed
  • Host species
    Mouse
  • Target species
    Human
  • Specificity
    hIgG1 specifically recognizes human IgG. hIgG - 100% hIgG1 - 147% hIgG2 - 150% hIgG3 - 94% hIgG4 - 2.1% hIgM - 0.6% hIgA - 0.3% Goat IgG - <0.01% Rabbit IgG - <0.01% Horse IgG - <0.01% Pig IgG - <0.01% Cow IgG - <0.01% Sheep IgG - <0.01% Affinity Constant: 2x1010 L/mol human IgG.
  • Tested applications
    Suitable for: ELISA, RIAmore details
  • Minimal
    cross-reactivity

    Cow, Goat, Horse, Pig, Rabbit, Sheep more details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 6.00
    Preservative: 0.095% Sodium azide
    Constituents: 0.335% PBS, 1.0878% Sodium citrate, 0.9% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    hIgG1
  • Isotype
    IgG2a
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
RIA
  • Application notes
    ELISA: Use at an assay dependant dilution.
    RIA: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References

    ab436 has not yet been referenced specifically in any publications.

    Publishing research using ab436? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-3 of 3 Abreviews or Q&A

    Question

    Hello,

    I am curious if any of the mouse anti-human IgG Mabs listed below cross react with monkey IgG?

    Mouse anti-Human IgG Fc secondary antibody (ab99757)

    Mouse monoclonal [H2] SecondaryAntibody to Human IgG - Fc (ab99770)

    Mouse anti-Human IgG1 Fc secondary antibody (ab1927)

    Mouse anti-Human IgG heavy chain secondary antibody (ab9243)

    Mouse monoclonal [hIgG1] SecondaryAntibody to Human IgG - H&L, pre-adsorbed (ab436)

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    Answer

    Thank you for contacting Abcam.

    We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, please see the link below for more details:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



    If there is anything else I can help you with, please let me know.

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    Question

    I am hunting for an antibody that will distinguish between intact human IgG and single F(ab). Does this monoclonal Distinguish between intact IgG antibody and the F(ab).

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    Answer

    It is unknown as to which constant region of the heavy chain this antibody recognises, so we do not know if this antibody could distinguish between the two.

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    Question

    Please advise if this antibody has cross reactivity with chimpanzee or not. Yours sincerely, Yoko Kurozumo

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    Answer

    We have not tested the cross reactivity of this antibody with chimpanzee or with any other monkey IgA or with other monkey Ig classes. Its cross reativity with human IgG is below 0.3% and with human IgM(plasma) 2.6%. Human IgM(Waldenström's macroglobulinemia) cross reacts less than 0.3%.

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