Overview

  • Product name

    Mouse monoclonal [hlgA1] Anti-Human IgA H&L
    See all IgA secondary antibodies
  • Description

    Mouse monoclonal [hlgA1] Anti-Human IgA H&L
  • Host species

    Mouse
  • Target species

    Human
  • Specificity

    hlgA1 specifically recognizes human IgA. hIgA - 100% hIgA (secretory) - 107% hIgA (myeloma) - 135% hIgA1 (myeloma) - 145% hIgA2 (myeloma) - 91% hIgA1 (myeloma) - 104% hIgA2 (myeloma) - 200% hIgA alpha-chain - 138% hIgG - <0.3% hIgG - <0.3% hIgM (plasma) - 2.6% hIgM (Waldenström's macroglobulinaemia) - <0.3% Affinity constant: 3x109 L/mol human plasma IgA.
  • Tested applications

    Suitable for: ELISA, RIAmore details

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6.00
    Preservative: 0.095% Sodium azide
    Constituents: 0.335% PBS, 1.0878% Sodium citrate, 0.9% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    hlgA1
  • Isotype

    IgG1
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.

References

This product has been referenced in:

  • Juhela S  et al. T cell responses to enterovirus antigens and to beta-cell autoantigens in unaffected children positive for IDDM-associated autoantibodies. J Autoimmun 12:269-78 (1999). Read more (PubMed: 10330298) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

Thank you for your enquiry. Ab 433 is the only IgA secondary antibody that we have. To see if another company may have what you're looking for, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249).

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Answer

We have not tested the cross reactivity of this antibody (Mouse monoclonal to human IgA) with monkey IgA or with other monkey Ig classes. Its cross reativity with human IgG is below 0.3% and with human IgM(plasma) 2.6%. Human IgM(Waldenström's macroglobulinemia) cross reacts less than 0.3%.

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Question
Answer

Anti-IgA (ab433) cross-reacted 107% with the secretory IgA preparation from DAKO. Cross reacivity with rat and saliva as a sample have not yet been tested.

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