Mouse monoclonal [HP6025] Anti-Human IgG4 Fc (ab99820)

Overview

  • Product name

    Mouse monoclonal [HP6025] Anti-Human IgG4 Fc
    See all IgG4 secondary antibodies
  • Host species

    Mouse
  • Target species

    Human
  • Specificity

    ab99820 reacts with the Fc portion of the heavy chain of IgG4.
  • Tested applications

    Suitable for: Dot blot, ELISA, WB, IHC-Fr, ICCmore details

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 8.20
    Constituent: 100% Borate buffered saline

    No preservatives or amine-containing buffer salts added.
  • Concentration information loading...
  • Purity

    Ascites
  • Purification notes

    Purified from ascites.
  • Clonality

    Monoclonal
  • Clone number

    HP6025
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution. for Dot and slot immunoblotting
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 36 kDa.
IHC-Fr Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

References

ab99820 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry.

I am sorry to confirm that epitope mapping has not been performed on this antibody. That being said, the following reference article states the epitope location is pFc’; therefore, theoretically it should be suitable for your protein.

Jefferis, R. et al. (1985) Evaluation of monoclonal antibodies having specificity for human IgG subclasses: results of an IVIS/WHO collaborative study. Immunology Letters. 10: 223252.

I am sorry this antibody has not been tested for cross reactivity with other species, though the below reference cites weak reactivity with goat IgG. It also states that this antibody does not cross react with rabbit, sheep, or horse IgG. Regrettably we were unable to locate reports regarding bovine IgG.

Reimer, C. B. et al. (1984) Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma. 3:263-275.

I hope this will be helpful. Please do not hesitate to let me know if you have any other questions.

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