Key features and details
- Mouse monoclonal [JDC-10] Anti-Human IgG Fc
- Host species: Mouse
- Isotype: IgG1
- Suitable for: ICC/IF, ELISA, WB, Dot blot, ICC, IHC-Fr
Product nameMouse monoclonal [JDC-10] Anti-Human IgG Fc
See all IgG secondary antibodies
Specificityab99757 reacts with the Fc portion of the heavy chain of all subclasses of Human IgG as demonstrated by ELISA; may also react with IgG from other species.
Tested applicationsSuitable for: ICC/IF, ELISA, WB, Dot blot, ICC, IHC-Frmore details
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 8.20
Constituent: 100% Borate buffered saline
No preservatives or amine-containing buffer salts added.
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab99757 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use 1µg for 106 cells. for indirect immunofluorescent staining of IgG+ Human B lymphocytes.|
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution.|
|Dot blot||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab99757 has been referenced in 1 publication.
- Chen SL et al. Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth. Exp Ther Med 12:2003-2008 (2016). PubMed: 27698685