Overview

  • Product name

    Mouse monoclonal [JDC-10] Anti-Human IgG Fc
    See all IgG secondary antibodies
  • Host species

    Mouse
  • Target species

    Human
  • Specificity

    ab99757 reacts with the Fc portion of the heavy chain of all subclasses of Human IgG as demonstrated by ELISA; may also react with IgG from other species.
  • Tested applications

    Suitable for: ICC/IF, ELISA, WB, Dot blot, ICC, IHC-Frmore details

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 8.20
    Constituent: 100% Borate buffered saline

    No preservatives or amine-containing buffer salts added.
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    JDC-10
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99757 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use 1µg for 106 cells. for indirect immunofluorescent staining of IgG+ Human B lymphocytes.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

References

This product has been referenced in:

  • Chen SL  et al. Targeting CRMP-4 by lentivirus-mediated RNA interference inhibits SW480 cell proliferation and colorectal cancer growth. Exp Ther Med 12:2003-2008 (2016). Read more (PubMed: 27698685) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting Abcam.

We have not tested any of those antibodies against monkey IgG, therefore I cannot say whether they will cross react or not. Due to this, you can take advantage of our testing discount program if you want, please see the link below for more details:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



If there is anything else I can help you with, please let me know.

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