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Immunology Immunoglobulins Heavy Chain IgG
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Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)

  • Datasheet
  • SDS
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ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)
  • ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)

Key features and details

  • Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP)
  • Conjugation: HRP
  • Host species: Mouse
  • Isotype: IgG1
  • Suitable for: ELISA, WB, IHC-Fr, Dot blot, ICC

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Overview

  • Product name

    Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP)
    See all IgG secondary antibodies
  • Host species

    Mouse
  • Target species

    Human
  • Specificity

    ab99759 reacts with the Fc portion of the heavy chain of all subclasses of Human IgG as demonstrated by ELISA; may also react with IgG from other species.
  • Tested applications

    Suitable for: ELISA, WB, IHC-Fr, Dot blot, ICCmore details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol, 50% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    JDC-10
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Immunoglobulins
    • Heavy Chain
    • IgG
    • Secondary antibodies
    • anti-Human
    • IgG
    • Enzyme
    • HRP

Associated products

  • Alternative Versions

    • Mouse monoclonal [JDC-10] Anti-Human IgG Fc (ab99757)
    • Mouse monoclonal [JDC-10] Anti-Human IgG Fc (Alkaline Phosphatase) (ab99758)
    • Mouse monoclonal [JDC-10] Anti-Human IgG heavy chain (Biotin) (ab99760)
    • Mouse monoclonal [JDC-10] Anti-Human IgG Fc (PE) (ab99761)

Applications

Our Abpromise guarantee covers the use of ab99759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/2000.
WB Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

Images

  • ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)
    ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)

    ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted ab99759.

  • ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)
    ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)

    ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted ab99759.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab99759? Please let us know so that we can cite the reference in this datasheet.

    ab99759 has been referenced in 2 publications.

    • Horie T  et al. Identification of OmpA-Like Protein of Tannerella forsythia as an O-Linked Glycoprotein and Its Binding Capability to Lectins. PLoS One 11:e0163974 (2016). ELISA . PubMed: 27711121
    • Akiyama S  et al. Prevalence of anti-phospholipase A2 receptor antibodies in Japanese patients with membranous nephropathy. Clin Exp Nephrol 19:653-60 (2015). WB . PubMed: 25412738

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