Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP)
See all IgG secondary antibodies -
Host species
Mouse -
Target species
Human -
Specificity
ab99759 reacts with the Fc portion of the heavy chain of all subclasses of Human IgG as demonstrated by ELISA; may also react with IgG from other species. -
Tested applications
Suitable for: ELISA, WB, IHC-Fr, Dot blot, ICCmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: None
Constituents: 50% PBS, 50% Glycerol, pH 7.4 -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
JDC-10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
Our Abpromise guarantee covers the use of ab99759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/1000 - 1/2000. | |
| WB | Use at an assay dependent dilution. | |
| IHC-Fr | Use at an assay dependent dilution. | |
| Dot blot | Use at an assay dependent dilution. | |
| ICC | Use at an assay dependent dilution. |
Images
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![ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)](https://www.abcam.com/ps/products/99/ab99759/Images/ab99759-331754-mouse-monoclonal-jdc-10-anti-human-igg-fc-hrp-elisa.jpg)
ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted ab99759.
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![ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)](https://www.abcam.com/ps/products/99/ab99759/Images/ab99759-331755-mouse-monoclonal-jdc-10-anti-human-igg-fc-hrp-elisa.jpg)
ELISA - Mouse monoclonal [JDC-10] Anti-Human IgG Fc (HRP) (ab99759)ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted ab99759.
References
This product has been referenced in:
- Horie T et al. Identification of OmpA-Like Protein of Tannerella forsythia as an O-Linked Glycoprotein and Its Binding Capability to Lectins. PLoS One 11:e0163974 (2016). ELISA . Read more (PubMed: 27711121) »
- Akiyama S et al. Prevalence of anti-phospholipase A2 receptor antibodies in Japanese patients with membranous nephropathy. Clin Exp Nephrol 19:653-60 (2015). WB . Read more (PubMed: 25412738) »
