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  1. Link

    mouse-monoclonal-jdc-12-human-lambda-light-chain-hrp-ab99811.pdf

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Immunology Immunoglobulins Light Chain Lambda
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Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP) (ab99811)

  • Datasheet
  • SDS
Reviews (1) Submit a question

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Key features and details

  • Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP)
  • Conjugation: HRP
  • Host species: Mouse
  • Isotype: IgG1
  • Suitable for: ELISA, Dot blot, ICC, WB, IHC-Fr

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Overview

  • Product name

    Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP)
    See all Lambda Light chain secondary antibodies
  • Host species

    Mouse
  • Target species

    Human
  • Specificity

    ab99811 reacts with lambda light chains as demonstrated by ELISA.
  • Tested applications

    Suitable for: ELISA, Dot blot, ICC, WB, IHC-Frmore details
  • Conjugation

    HRP

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.40
    Constituents: 50% Glycerol, 50% PBS
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    JDC-12
  • Isotype

    IgG1
  • Light chain type

    kappa
  • General notes

     

     

  • Research areas

    • Immunology
    • Immunoglobulins
    • Light Chain
    • Lambda
    • Secondary antibodies
    • anti-Human
    • Lambda light chain
    • Enzyme
    • HRP

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab99811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA (1)
1/1000 - 1/2000.
Dot blot
Use at an assay dependent dilution.
ICC
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.
Notes
ELISA
1/1000 - 1/2000.
Dot blot
Use at an assay dependent dilution.
ICC
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab99811? Please let us know so that we can cite the reference in this datasheet.

ab99811 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Detecting humanIgG1/Lambda binding to humanIgG-Fc Fusion protein

Excellent
Abreviews
Abreviews
abreview image
Application
ELISA
This reagent is indispensable when detecting binding of a humanIgG1/lambda antibody to antigen that is fused to humanIgG1 in a direct ELISA. Coating antigen at 10ug/ml, antibody at 1ug/ml, and diluting this reagent 1:1000 gave sufficient signal for detection with low background.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 04 2018

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