Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP) (ab99811)

Overview

  • Product name
    Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP)
    See all Lambda Light chain secondary antibodies
  • Host species
    Mouse
  • Target species
    Human
  • Specificity
    ab99811 reacts with lambda light chains as demonstrated by ELISA.
  • Tested applications
    Suitable for: ELISA, Dot blot, ICC, WB, IHC-Frmore details
  • Conjugation
    HRP

Properties

Applications

Our Abpromise guarantee covers the use of ab99811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000 - 1/2000.
Dot blot Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.

References

ab99811 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
ELISA
This reagent is indispensable when detecting binding of a humanIgG1/lambda antibody to antigen that is fused to humanIgG1 in a direct ELISA. Coating antigen at 10ug/ml, antibody at 1ug/ml, and diluting this reagent 1:1000 gave sufficient signal for detection with low background.

Abcam user community

Verified customer

Submitted Oct 04 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up