Overview

  • Product name
    Mouse MPO ELISA Kit (Myeloperoxidase)
    See all Myeloperoxidase kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Plasma, Cell Lysate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 0.6 ng/ml
  • Range
    0.61 ng/ml - 150 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 105.5 96% - 112%
    Plasma 86.89 68% - 105%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s MPO (Myeloperoxidase) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse MPO (Myeloperoxidase) in plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse MPO coated on a 96-well plate. Standards and samples are pipetted into the wells and MPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.


    We have not been able to detect the endogenous Mouse MPO (Myeloperoxidase) in normal serum with ab155458, only in serum spiked with Mouse MPO (Myeloperoxidase).

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab155458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab155458 - Assay Diluent B.

  • Representative standard curve using ab155458 - Assay Diluent C.

Protocols

References

This product has been referenced in:
  • Tam BT  et al. Ablation of Bax and Bak protects skeletal muscle against pressure-induced injury. Sci Rep 8:3689 (2018). Read more (PubMed: 29487339) »
  • Ding D  et al. 3-Methyladenine and dexmedetomidine reverse lipopolysaccharide-induced acute lung injury through the inhibition of inflammation and autophagy. Exp Ther Med 15:3516-3522 (2018). Read more (PubMed: 29545877) »
See all 8 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

MPO in mice colon tissue

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Samples were from mice (Balb/C) colon tissue, homogenized in a non-ionic RIPA-buffer using a Precellys homogenizer. Protein concentration was determined using BCA-Assay. 250 µg protein was used in the MPO assay (normalisation with lysisbuffer B). All sample, standards and controls were assay in duplicates. Incubation of standard and samples over night at 4°C with gentle shaking.
The standard curve was as described as the typical curve. The highest concentration was eliminated, because the absorbance was not detectable anymore. The standard curve display a R²=0,873.

Miss. Anne Breitrueck

Verified customer

Submitted Aug 12 2014

skin wound sample

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Crashed murine skin wound sample and added lysis buffer. Filtrated-samples were measured with MPO ELISA kit (ab155458) in order to investigate neutrophil infiltration at wound site. I could get nice results.

Abcam user community

Verified customer

Submitted Sep 03 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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