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Immunology Innate Immunity Macrophage / Inflamm.
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Mouse MPO ELISA Kit (Myeloperoxidase) (ab155458)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (2) Submit a question References (13)

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Typical Standard Curve
  • Typical Standard Curve

Key features and details

  • Sensitivity: 0.6 ng/ml
  • Range: 0.61 ng/ml - 150 ng/ml
  • Sample type: Cell culture supernatant, Cell Lysate, Plasma
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse MPO ELISA Kit (Myeloperoxidase)
    See all Myeloperoxidase kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Plasma, Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 0.6 ng/ml
  • Range

    0.61 ng/ml - 150 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 105.5 96% - 112%
    Plasma 86.89 68% - 105%
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Abcam’s MPO (Myeloperoxidase) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse MPO (Myeloperoxidase) in plasma and cell culture supernatants.


    This assay employs an antibody specific for mouse MPO coated on a 96-well plate. Standards and samples are pipetted into the wells and MPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.


    We have not been able to detect the endogenous Mouse MPO (Myeloperoxidase) in normal serum with ab155458, only in serum spiked with Mouse MPO (Myeloperoxidase).

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent C 1 x 30ml
    Biotinylated anti-mouse MPO 2 vials
    MPO Microplate (12 x 8 wells) 1 unit
    Recombinant Mouse MPO Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Cardiovascular
    • Blood
    • Other
    • Cardiovascular
    • Vasculature
    • Vasculature Markers
    • Arterial
    • Cardiovascular
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    • Cancer Metabolism
    • Cellular metabolic process
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    • Redox metabolism
    • Antioxidants
  • Function

    Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
  • Involvement in disease

    Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
  • Sequence similarities

    Belongs to the peroxidase family. XPO subfamily.
  • Cellular localization

    Lysosome.
  • Target information above from: UniProt accession P05164 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 84 kDa myeloperoxidase
    • 89 kDa myeloperoxidase
    • EC 1.11.1.7
    • EC1.11.2.2
    • fj80f04
    • MPO
    • mpx
    • myeloid-specific peroxidase
    • Myeloperoxidase
    • Myeloperoxidase heavy chain
    • Myeloperoxidase light chain
    • PERM_HUMAN
    • wu:fj80f04
    see all
  • Database links

    • Entrez Gene: 17523 Mouse
    • SwissProt: P11247 Mouse
    • Unigene: 4668 Mouse

    Associated products

      Images

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab155458 - Assay Diluent B.

      • Typical Standard Curve
        Typical Standard Curve

        Representative standard curve using ab155458 - Assay Diluent C.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (13)

      Publishing research using ab155458? Please let us know so that we can cite the reference in this datasheet.

      ab155458 has been referenced in 13 publications.

      • Zhang Y  et al. Selenomethionine supplementation reduces lesion burden, improves vessel function and modulates the inflammatory response within the setting of atherosclerosis. Redox Biol 29:101409 (2020). PubMed: 31926617
      • Dikshit N  et al. NLRP3 inflammasome pathway has a critical role in the host immunity against clinically relevant Acinetobacter baumannii pulmonary infection. Mucosal Immunol 11:257-272 (2018). PubMed: 28612844
      • Tam BT  et al. Ablation of Bax and Bak protects skeletal muscle against pressure-induced injury. Sci Rep 8:3689 (2018). PubMed: 29487339
      • Ding D  et al. 3-Methyladenine and dexmedetomidine reverse lipopolysaccharide-induced acute lung injury through the inhibition of inflammation and autophagy. Exp Ther Med 15:3516-3522 (2018). PubMed: 29545877
      • Mishra OP  et al. Synthetic secoisolariciresinol diglucoside (LGM2605) inhibits myeloperoxidase activity in inflammatory cells. Biochim Biophys Acta 1862:1364-1375 (2018). PubMed: 29524540
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      MPO in mice colon tissue

      Excellent Excellent 5/5 (Ease of Use)
      Abreviews
      Abreviews
      Samples were from mice (Balb/C) colon tissue, homogenized in a non-ionic RIPA-buffer using a Precellys homogenizer. Protein concentration was determined using BCA-Assay. 250 µg protein was used in the MPO assay (normalisation with lysisbuffer B). All sample, standards and controls were assay in duplicates. Incubation of standard and samples over night at 4°C with gentle shaking.
      The standard curve was as described as the typical curve. The highest concentration was eliminated, because the absorbance was not detectable anymore. The standard curve display a R²=0,873.

      Miss. Anne Breitrueck

      Verified customer

      Submitted Aug 12 2014

      skin wound sample

      Excellent Excellent 5/5 (Ease of Use)
      Abreviews
      Abreviews
      Crashed murine skin wound sample and added lysis buffer. Filtrated-samples were measured with MPO ELISA kit (ab155458) in order to investigate neutrophil infiltration at wound site. I could get nice results.

      Abcam user community

      Verified customer

      Submitted Sep 03 2013

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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