Mouse MPO ELISA Kit (Myeloperoxidase) (ab155458)
Key features and details
- Sensitivity: 0.6 ng/ml
- Range: 0.61 ng/ml - 150 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MPO ELISA Kit (Myeloperoxidase)
See all Myeloperoxidase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Plasma, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 0.6 ng/ml -
Range
0.61 ng/ml - 150 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 105.5 96% - 112% Plasma 86.89 68% - 105% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s MPO (Myeloperoxidase) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse MPO (Myeloperoxidase) in plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MPO coated on a 96-well plate. Standards and samples are pipetted into the wells and MPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.
We have not been able to detect the endogenous Mouse MPO (Myeloperoxidase) in normal serum with ab155458, only in serum spiked with Mouse MPO (Myeloperoxidase).
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-mouse MPO 2 vials MPO Microplate (12 x 8 wells) 1 unit Recombinant Mouse MPO Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. -
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis. -
Sequence similarities
Belongs to the peroxidase family. XPO subfamily. -
Cellular localization
Lysosome. - Information by UniProt
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Alternative names
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 1.11.1.7
see all -
Database links
- Entrez Gene: 17523 Mouse
- SwissProt: P11247 Mouse
- Unigene: 4668 Mouse
Images
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Typical Standard Curve
Representative standard curve using ab155458 - Assay Diluent B.
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Typical Standard CurveRepresentative standard curve using ab155458 - Assay Diluent C.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab155458 has been referenced in 15 publications.
- Zhang Y et al. Selenomethionine supplementation reduces lesion burden, improves vessel function and modulates the inflammatory response within the setting of atherosclerosis. Redox Biol 29:101409 (2020). PubMed: 31926617
- Smith P et al. Effects of different routes of endotoxin injury on barrier function in alcoholic lung syndrome. Alcohol 80:81-89 (2019). PubMed: 31278041
- Sun Y et al. Complex TNF-a B cell epitope MAP vaccine alleviates murine ulcerative colitis. Int J Mol Med 44:1106-1116 (2019). PubMed: 31524230
- Dikshit N et al. NLRP3 inflammasome pathway has a critical role in the host immunity against clinically relevant Acinetobacter baumannii pulmonary infection. Mucosal Immunol 11:257-272 (2018). PubMed: 28612844
- Tam BT et al. Ablation of Bax and Bak protects skeletal muscle against pressure-induced injury. Sci Rep 8:3689 (2018). PubMed: 29487339