Mouse MPO ELISA Kit (Myeloperoxidase) (ab155458)
- Datasheet
- References (9)
- Protocols
Overview
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Product nameMouse MPO ELISA Kit (Myeloperoxidase)
See all Myeloperoxidase kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample typeCell culture supernatant, Plasma, Cell Lysate
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Assay typeSandwich (quantitative)
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Sensitivity< 0.6 ng/ml
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Range0.61 ng/ml - 150 ng/ml
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Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 105.5 96% - 112% Plasma 86.89 68% - 105% -
Assay durationMultiple steps standard assay
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Species reactivityReacts with: Mouse
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Product overview
Abcam’s MPO (Myeloperoxidase) Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of mouse MPO (Myeloperoxidase) in plasma and cell culture supernatants.
This assay employs an antibody specific for mouse MPO coated on a 96-well plate. Standards and samples are pipetted into the wells and MPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse MPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm.
We have not been able to detect the endogenous Mouse MPO (Myeloperoxidase) in normal serum with ab155458, only in serum spiked with Mouse MPO (Myeloperoxidase).
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-mouse MPO 2 vials MPO Microplate (12 x 8 wells) 1 unit Recombinant Mouse MPO Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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FunctionPart of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.
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Involvement in diseaseDefects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an autosomal recessive defect that results in disseminated candidiasis.
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Sequence similaritiesBelongs to the peroxidase family. XPO subfamily.
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Cellular localizationLysosome.
- Information by UniProt
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Alternative names
- 84 kDa myeloperoxidase
- 89 kDa myeloperoxidase
- EC 1.11.1.7
see all -
Database links
- Entrez Gene: 17523 Mouse
- SwissProt: P11247 Mouse
- Unigene: 4668 Mouse
Applications
Our Abpromise guarantee covers the use of ab155458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative standard curve using ab155458 - Assay Diluent B.
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Typical Standard CurveRepresentative standard curve using ab155458 - Assay Diluent C.
Protocols
References
This product has been referenced in:
- Tam BT et al. Ablation of Bax and Bak protects skeletal muscle against pressure-induced injury. Sci Rep 8:3689 (2018). Read more (PubMed: 29487339) »
- Ding D et al. 3-Methyladenine and dexmedetomidine reverse lipopolysaccharide-induced acute lung injury through the inhibition of inflammation and autophagy. Exp Ther Med 15:3516-3522 (2018). Read more (PubMed: 29545877) »
