Samples were from mice (Balb/C) colon tissue, homogenized in a non-ionic RIPA-buffer using a Precellys homogenizer. Protein concentration was determined using BCA-Assay. 250 µg protein was used in the MPO assay (normalisation with lysisbuffer B). All sample, standards and controls were assay in duplicates. Incubation of standard and samples over night at 4°C with gentle shaking.
The standard curve was as described as the typical curve. The highest concentration was eliminated, because the absorbance was not detectable anymore. The standard curve display a R²=0,873.
The standard curve was as described as the typical curve. The highest concentration was eliminated, because the absorbance was not detectable anymore. The standard curve display a R²=0,873.
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MISS. Anne Breitrueck
Verified customer
Submitted Aug 12 2014