Mouse MSP ELISA Kit (MST1) (ab213879)

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Overview

  • Product name

    Mouse MSP ELISA Kit (MST1)

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Sample 1 16 927pg/ml 53.77 5.8%
    Sample 2 16 2438pg/ml 134.1 5.5%
    Sample 3 16 5438pg/ml 234 4.3%
    Inter-assay
    Sample n Mean SD CV%
    Sample 1 24 1241pg/ml 83.15 6.7%
    Sample 2 24 2655pg/ml 162 6.1%
    Sample 3 24 5632pg/ml 366 6.5%

  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    < 10 pg/ml

  • Range

    156 pg/ml - 10000 pg/ml

  • Assay time

    3h 30m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    The Mouse MSP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213879) is designed for the quantitative measurement of Mouse MSP in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for MSP/MST1 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: Q19-E716) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MSP/MST1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse MSP/MST1 amount of sample captured in plate.

  • Notes

    Macrophage Stimulating 1 (MST1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21). The RON tyrosine kinase, the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Mouse MSP coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Mouse MSP antibody 1 x 130µl
    Lyophilized recombinant Mouse MSP standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml

  • Research areas

  • Function

    Probably has no proteolytic activity, since crucial characteristic of serine proteases catalytic sites are not conserved.

  • Sequence similarities

    Belongs to the peptidase S1 family. Plasminogen subfamily.
    Contains 4 kringle domains.
    Contains 1 PAN domain.
    Contains 1 peptidase S1 domain.

  • Post-translational
    modifications

    May be cleaved after Arg-483, to yield two chains held together by disulfide bonds, or two separate polypeptides.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • D3F15S2
    • DNF15S2
    • Hepatocyte growth factor like protein
    • Hepatocyte growth factor like protein alpha chain
    • Hepatocyte growth factor like protein beta chain
    • Hepatocyte growth factor like protein homolog
    • Hepatocyte growth factor-like protein beta chain
    • HGFL
    • HGFL_HUMAN
    • Macrophage stimulating 1 (hepatocyte growth factor like)
    • Macrophage stimulatory protein
    • Macrophage-stimulating protein
    • MSP
    • MST1
    • NF15S2
    • OTTHUMP00000208927
    see all

  • Database links

    Applications

    Our Abpromise guarantee covers the use of ab213879 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA Use at an assay dependent concentration.

    Images

    • Mouse MSP ELISA Kit (ab213879) Standard Curve
      Mouse MSP ELISA Kit (ab213879) Standard Curve

      Mouse MSP ELISA Kit (ab213879) Standard Curve.

    Protocols

    References

    This product has been referenced in:

    • Yu J  et al. Identification of MST1 as a potential early detection biomarker for colorectal cancer through a proteomic approach. Sci Rep 7:14265 (2017). Read more (PubMed: 29079854) »
    See 1 Publication for this product

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