Mouse MSP ELISA Kit (MST1) (ab213879)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse MSP ELISA Kit (MST1) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 257pg/ml 11.56 = 4.5% 2 16 1513pg/ml 62.03 = 4.1% 3 16 5860pg/ml 322.3 = 5.5% Inter-assay Sample n Mean SD CV% 1 24 276pg/ml 12.97 = 4.7% 2 24 1456pg/ml 61.15 = 4.2% 3 24 5996pg/ml 425.71 = 7.1% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse MSP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213879) is designed for the quantitative measurement of Mouse MSP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for MSP/MST1 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: Q19-E716) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MSP/MST1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse MSP/MST1 amount of sample captured in plate.
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Notes
Macrophage Stimulating 1 (MST1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21). The RON tyrosine kinase, the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse MSP coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti- Mouse MSP antibody 1 x 130µl Lyophilized recombinant Mouse MSP standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Probably has no proteolytic activity, since crucial characteristic of serine proteases catalytic sites are not conserved. -
Sequence similarities
Belongs to the peptidase S1 family. Plasminogen subfamily.
Contains 4 kringle domains.
Contains 1 PAN domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsMay be cleaved after Arg-483, to yield two chains held together by disulfide bonds, or two separate polypeptides. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- D3F15S2
- DNF15S2
- Hepatocyte growth factor like protein
see all -
Database links
- Entrez Gene: 15235 Mouse
- SwissProt: P26928 Mouse
- Unigene: 8369 Mouse
Images
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Mouse MSP ELISA Kit (ab213879) Standard Curve
Mouse MSP ELISA Kit (ab213879) Standard Curve.
References (1)
ab213879 has been referenced in 1 publication.
- Yu J et al. Identification of MST1 as a potential early detection biomarker for colorectal cancer through a proteomic approach. Sci Rep 7:14265 (2017). PubMed: 29079854
