For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    mouse-msp-elisa-kit-mst1-ab213879.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Innate Immunity Macrophage / Inflamm.
Share by email

Mouse MSP ELISA Kit (MST1) (ab213879)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Mouse MSP ELISA Kit (ab213879) Standard Curve

    Key features and details

    • Sensitivity: 10 pg/ml
    • Range: 156 pg/ml - 10000 pg/ml
    • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Mouse

    Overview

    • Product name

      Mouse MSP ELISA Kit (MST1)
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      1 16 257pg/ml 11.56 = 4.5%
      2 16 1513pg/ml 62.03 = 4.1%
      3 16 5860pg/ml 322.3 = 5.5%
      Inter-assay
      Sample n Mean SD CV%
      1 24 276pg/ml 12.97 = 4.7%
      2 24 1456pg/ml 61.15 = 4.2%
      3 24 5996pg/ml 425.71 = 7.1%
    • Sample type

      Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      < 10 pg/ml
    • Range

      156 pg/ml - 10000 pg/ml
    • Assay time

      3h 30m
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Mouse
    • Product overview

      The Mouse MSP Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213879) is designed for the quantitative measurement of Mouse MSP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).


      The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for MSP/MST1 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: Q19-E716) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MSP/MST1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse MSP/MST1 amount of sample captured in plate.

    • Notes

      Macrophage Stimulating 1 (MST1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21). The RON tyrosine kinase, the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 1 x 96 tests
      ABC Diluent Buffer Blue Cap 1 x 12ml
      Adhesive Plate Seal 4 units
      Antibody Diluent Buffer Green Cap 1 x 12ml
      Anti-Mouse MSP coated Microplate (12 x 8 wells) 1 unit
      Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
      Biotinylated anti- Mouse MSP antibody 1 x 130µl
      Lyophilized recombinant Mouse MSP standard 2 vials
      Sample Diluent Buffer Green Cap 1 x 30ml
      TMB Color Developing Agent Black Cap 1 x 10ml
      TMB Stop Solution Yellow Cap 1 x 10ml
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Cancer
      • Tumor biomarkers
      • Other
    • Function

      Probably has no proteolytic activity, since crucial characteristic of serine proteases catalytic sites are not conserved.
    • Sequence similarities

      Belongs to the peptidase S1 family. Plasminogen subfamily.
      Contains 4 kringle domains.
      Contains 1 PAN domain.
      Contains 1 peptidase S1 domain.
    • Post-translational
      modifications

      May be cleaved after Arg-483, to yield two chains held together by disulfide bonds, or two separate polypeptides.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession P26927 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Alternative names

      • D3F15S2
      • DNF15S2
      • Hepatocyte growth factor like protein
      • Hepatocyte growth factor like protein alpha chain
      • Hepatocyte growth factor like protein beta chain
      • Hepatocyte growth factor like protein homolog
      • Hepatocyte growth factor-like protein beta chain
      • HGFL
      • HGFL_HUMAN
      • Macrophage stimulating 1 (hepatocyte growth factor like)
      • Macrophage stimulatory protein
      • Macrophage-stimulating protein
      • MSP
      • MST1
      • NF15S2
      • OTTHUMP00000208927
      see all
    • Database links

      • Entrez Gene: 15235 Mouse
      • SwissProt: P26928 Mouse
      • Unigene: 8369 Mouse

      Images

      • Mouse MSP ELISA Kit (ab213879) Standard Curve
        Mouse MSP ELISA Kit (ab213879) Standard Curve

        Mouse MSP ELISA Kit (ab213879) Standard Curve.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab213879? Please let us know so that we can cite the reference in this datasheet.

      ab213879 has been referenced in 1 publication.

      • Yu J  et al. Identification of MST1 as a potential early detection biomarker for colorectal cancer through a proteomic approach. Sci Rep 7:14265 (2017). PubMed: 29079854

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab213879.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.