Overview

  • Product name

    Mouse mTOR Matched Antibody Pair Kit
    See all mTOR kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    62.5 pg/ml - 4000 pg/ml
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Product overview

    Matched Antibody Pair kit is a titrated unlabeled capture antibody, a titrated biotin-labeled detector and a calibrated protein standard. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse mTOR. 


    Both capture and detector antibodies are rabbit monoclonal antibodies.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification. 


    Additional protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab206311) which uses the same antibody pair. 


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.


    Buffer information:
    The capture antibody is glycerol free. The detector antibody contains glycerol.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Mouse mTOR Capture Antibody 1 x 100µg 1 x 20µg
    Mouse mTOR Detector Antibody 1 x 25µg 1 x 5µg
    Mouse mTOR Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
  • Tissue specificity

    Expressed in numerous tissues, with highest levels in testis.
  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 7 HEAT repeats.
    Contains 1 PI3K/PI4K domain.
  • Post-translational
    modifications

    Autophosphorylated; when part of mTORC1 or mTORC2.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
  • Information by UniProt
  • Alternative names

    • dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1)
    • FK506 binding protein 12 rapamycin associated protein 1
    • FK506 binding protein 12 rapamycin associated protein 2
    • FK506 binding protein 12 rapamycin complex associated protein 1
    • FK506-binding protein 12-rapamycin complex-associated protein 1
    • FKBP rapamycin associated protein
    • FKBP12 rapamycin complex associated protein
    • FKBP12-rapamycin complex-associated protein
    • FKBP12-rapamycin complex-associated protein 1
    • FLJ44809
    • FRAP
    • FRAP1
    • FRAP2
    • Mammalian target of rapamycin
    • Mechanistic target of rapamycin
    • mTOR
    • MTOR_HUMAN
    • OTTHUMP00000001983
    • RAFT1
    • Rapamycin and FKBP12 target 1
    • Rapamycin associated protein FRAP2
    • Rapamycin target protein
    • Rapamycin target protein 1
    • RAPT1
    • Serine/threonine-protein kinase mTOR
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab215406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background subtracted values are graphed.

     

Protocols

References

ab215406 has not yet been referenced specifically in any publications.

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