Key features and details
- Sensitivity: 4.48 ng/ml
- Range: 9.375 ng/ml - 300 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse Myoglobin ELISA Kit
See all Myoglobin kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10%
Sample typeSerum, Plasma
Assay typeSandwich (quantitative)
Range9.375 ng/ml - 300 ng/ml
Sample specific recovery Sample type Average % Range Serum > 85 % - %
Assay durationMultiple steps standard assay
Species reactivityReacts with: Mouse
Abcam’s Myoglobin Mouse ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Myoglobin in mouse samples.
In this assay the Myoglobin present in samples reacts with the anti-Myoglobin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Myoglobin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound Myoglobin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Myoglobin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Myoglobin in the test sample. The quantity of Myoglobin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Get better reproducibility in only 90 minutes with Mouse Myoglobin ELISA Kit (ab210965) from our SimpleStep ELISA® range.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 100X HRP-conjugated anti-mouse Myoglobin antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Mouse Myoglobin Calibrator (Lyophilized) 1 vial Mouse Myoglobin ELISA Microplate 1 unit Stop Solution 1 x 12ml
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
FunctionServes as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
Sequence similaritiesBelongs to the globin family.
- Information by UniProt
SimpleStep ELISA kits
ab157722 has been referenced in 1 publication.
- Warfel JD et al. A low fat diet ameliorates pathology but retains beneficial effects associated with CPT1b knockout in skeletal muscle. PLoS One 12:e0188850 (2017). ELISA ; Mouse . PubMed: 29240830