Overview

  • Product name

    Mouse Myoglobin ELISA Kit
    See all Myoglobin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 6.1%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 4.8%
  • Sample type

    Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    9.4 pg/ml
  • Range

    15.62 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 115 110% - 123%
    Serum 88 76% - 99%
    Tissue Extracts 112 108% - 119%
    Heparin Plasma 105 100% - 113%
    EDTA Plasma 100 95% - 106%
    Citrate Plasma 112 100% - 120%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
    Does not react with: Cow, Human
  • Product overview

    Myoglobin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Myoglobin protein in mouse serum, plasma, and cell culture supernatant, cell and tissue extract samples.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material.  TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


    Sensitivity:
    Samples diluted in Sample Diluent NS MDD =10.6 pg/mL
    Samples diluted in 1X Cell Extraction Buffer PTR MDD = 9.4 pg/mL

  • Notes

    Myoglobin is an oxygen- and iron-binding protein involved in the transportation of oxygen within muscles. Upon muscle injury, myoglobin can also be found in the bloodstream.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab210965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 37.5%, plasma (heparin) 20%, plasma (EDTA) 25% and heart supernatant 1:1600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 6.87 ng/mL in serum, 3.57 ng/mL in plasma (citrate), 6.35 ng/mL in plasma (heparin), 7.76 ng/mL in plasma (EDTA) and 4,187 ng/mL in heart supernatant.

  • The concentrations of Myoglobin were measured in duplicate and interpolated from the Myoglobin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 1.08 ng/mL in skeletal muscle extract and 0.67 ng/mL in C2C12 extract.

Protocols

References

ab210965 has not yet been referenced specifically in any publications.

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