Mouse Myoglobin ELISA Kit (ab210965)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse Myoglobin ELISA Kit
See all Myoglobin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.1% Inter-assay Sample n Mean SD CV% Serum 3 4.8% -
Sample type
Cell culture supernatant, Serum, Plasma, Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
9.4 pg/ml -
Range
15.62 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 115 110% - 123% Serum 88 76% - 99% Tissue Extracts 112 108% - 119% Heparin Plasma 105 100% - 113% EDTA Plasma 100 95% - 106% Citrate Plasma 112 100% - 120% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow, Human -
Product overview
Myoglobin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Myoglobin protein in mouse serum, plasma, and cell culture supernatant, cell and tissue extract samples.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Sensitivity:
Samples diluted in Sample Diluent NS MDD =10.6 pg/mL
Samples diluted in 1X Cell Extraction Buffer PTR MDD = 9.4 pg/mL -
Notes
Myoglobin is an oxygen- and iron-binding protein involved in the transportation of oxygen within muscles. Upon muscle injury, myoglobin can also be found in the bloodstream.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Myoglobin Capture Antibody 1 x 600µl 10X Mouse Myoglobin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Mouse Myoglobin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. -
Sequence similarities
Belongs to the globin family. - Information by UniProt
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Alternative names
- MB
- MGC13548
- MYG_HUMAN
- Myoglobin
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Database links
- Entrez Gene: 17189 Mouse
- SwissProt: P04247 Mouse
- Unigene: 404074 Mouse
Associated products
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ELISA kits
Applications
Our Abpromise guarantee covers the use of ab210965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Other - Mouse Myoglobin ELISA Kit (ab210965)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Myoglobin standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse Myoglobin standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Myoglobin in mouse serum, plasma and cell culture supernatant samples.The concentrations of Myoglobin were measured in duplicates, interpolated from the Myoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 37.5%, plasma (heparin) 20%, plasma (EDTA) 25% and heart supernatant 1:1600. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 6.87 ng/mL in serum, 3.57 ng/mL in plasma (citrate), 6.35 ng/mL in plasma (heparin), 7.76 ng/mL in plasma (EDTA) and 4,187 ng/mL in heart supernatant.
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Interpolated concentrations of native Myoglobin in mouse skeletal muscle based on a 300 ng/mL extract load and C2C12 extract based on a 125 µg/mL extract load.The concentrations of Myoglobin were measured in duplicate and interpolated from the Myoglobin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Myoglobin concentration was determined to be 1.08 ng/mL in skeletal muscle extract and 0.67 ng/mL in C2C12 extract.
Protocols
References
ab210965 has not yet been referenced specifically in any publications.
