Key features and details
- One-wash 90 minute protocol
- Sensitivity: 21.6 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Product nameMouse NBL1 ELISA Kit
See all NBL1 kits
Intra-assay Sample n Mean SD CV% Protein 8 3.9%
Assay typeSandwich (quantitative)
Range62.5 pg/ml - 4000 pg/ml
Sample specific recovery Sample type Average % Range Serum 101 % - %
Assay time1h 30m
Assay durationOne step assay
Species reactivityReacts with: Mouse
Mouse NBL1 ELISA Kit (ab234575) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NBL1 protein in serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse NBL1 with 21.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Mouse NBL1 Capture Antibody 1 x 600µl 10X Mouse NBL1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent CPR 1 x 6ml Mouse NBL1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml
- Information by UniProt
- DAN domain family member 1
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Background-subtracted data values (mean +/- SD) are graphed.
The concentrations of NBL1 were measured in duplicates, interpolated from the NBL1 standard curves and corrected for sample dilution. Samples were loaded at 4% serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NBL1 concentration was determined to be 63.4 ng/mL in mouse serum.
ab234575 has not yet been referenced specifically in any publications.