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    mouse-nestin-elisa-kit-ab223859.pdf

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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments
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RecombinantSimpleStep

Mouse Nestin ELISA Kit (ab223859)

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Other - Mouse Nestin ELISA Kit (ab223859)
  • Example of MouseNestin standard curve in 1X Cell Extraction Buffer PTR
  • Interpolated concentrations of native  Nestin in Mouse NIH/3T3 cell extract sample and sample based on a 62.5 µg/mL extract load
  • Sandwich ELISA - Mouse Nestin ELISA Kit (ab223859)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 7.6 pg/ml
  • Range: 15.6 pg/ml - 1000 pg/ml
  • Sample type: Cell culture extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse Nestin ELISA Kit
    See all Nestin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Cell Extract 5 3.4%
    Inter-assay
    Sample n Mean SD CV%
    Cell Extract 3 14.1%
  • Sample type

    Cell culture extracts
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    7.6 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 107 103% - 112%
  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    Mouse Nestin ELISA Kit (ab223859) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nestin protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Nestin with 7.6 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Nestin is an intermediate filament protein and is a marker for neural stem cells.  Nestin is required for brain and eye development.   Nestin expression generally doesn’t persist into adult hood, with the exception of the neuronal precursor cells of the subgranular zone.  After cells have differentiated Nestin is downregulated and is replaced by tissue-specific intermediate filament proteins.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse Nestin Capture Antibody 1 x 600µl
    10X Mouse Nestin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPR 1 x 6ml
    Mouse Nestin Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Tumor antigens
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Other
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Neuroscience
    • Development
  • Function

    Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
  • Tissue specificity

    CNS stem cells.
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Developmental stage

    Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
  • Post-translational
    modifications

    Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
  • Target information above from: UniProt accession P48681 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • ESTM 46
    • FLJ 21841
    • FLJ21841
    • Intermediate filament protein
    • Nbla00170
    • nes
    • NEST_HUMAN
    • Nestin
    see all
  • Database links

    • Entrez Gene: 18008 Mouse
    • SwissProt: Q6P5H2 Mouse
    • Unigene: 331129 Mouse

    Associated products

    • Alternative Versions

      • Mouse Nestin Antibody Pair - BSA and Azide free (ab244019)

    Images

    • Other - Mouse Nestin ELISA Kit (ab223859)
      Other - Mouse Nestin ELISA Kit (ab223859)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of MouseNestin standard curve in 1X Cell Extraction Buffer PTR
      Example of MouseNestin standard curve in 1X Cell Extraction Buffer PTR

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native  Nestin in Mouse NIH/3T3 cell extract sample and sample based on a 62.5 µg/mL extract load
      Interpolated concentrations of native Nestin in Mouse NIH/3T3 cell extract sample and sample based on a 62.5 µg/mL extract load

      The concentrations of Nestin were measured in duplicate and interpolated from the Nestin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nestin concentration was determined to be 678 pg/mL in NIH/3T3 Cell extract.

    • Sandwich ELISA - Mouse Nestin ELISA Kit (ab223859)
      Sandwich ELISA - Mouse Nestin ELISA Kit (ab223859)
      To learn more about the advantages of recombinant antibodies see here.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

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    ab223859 has not yet been referenced specifically in any publications.

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