Mouse Nestin ELISA Kit (ab223859)
- Datasheet
- References
- Protocols
Overview
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Product name
Mouse Nestin ELISA Kit
See all Nestin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell Extract 5 3.4% Inter-assay Sample n Mean SD CV% Cell Extract 3 14.1% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
7.6 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 107 103% - 112% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Nestin in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Nestin protein in Mouse cell extracts.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Nestin is an intermediate filament protein and is a marker for neural stem cells. Nestin is required for brain and eye development. Nestin expression generally doesn’t persist into adult hood, with the exception of the neuronal precursor cells of the subgranular zone. After cells have differentiated Nestin is downregulated and is replaced by tissue-specific intermediate filament proteins.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 12ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml 10X Mouse Nestin Capture Antibody 1 x 600µl 10X Mouse Nestin Detector Antibody 1 x 600µl Mouse Nestin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. -
Tissue specificity
CNS stem cells. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. -
Post-translational
modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. - Information by UniProt
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Alternative names
- ESTM 46
- FLJ 21841
- FLJ21841
see all -
Database links
- Entrez Gene: 18008 Mouse
- SwissProt: Q6P5H2 Mouse
- Unigene: 331129 Mouse
Associated products
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Alternative Versions
Images
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Other - Mouse Nestin ELISA Kit (ab223859)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of MouseNestin standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Nestin in Mouse NIH/3T3 cell extract sample and sample based on a 62.5 µg/mL extract loadThe concentrations of Nestin were measured in duplicate and interpolated from the Nestin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nestin concentration was determined to be 678 pg/mL in NIH/3T3 Cell extract.
Protocols
References
ab223859 has not yet been referenced specifically in any publications.
