Mouse Nestin ELISA Kit (ab223859)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.6 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Nestin ELISA Kit
See all Nestin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Cell Extract 5 3.4% Inter-assay Sample n Mean SD CV% Cell Extract 3 14.1% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
7.6 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 107 103% - 112% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Nestin ELISA Kit (ab223859) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nestin protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Nestin with 7.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Nestin is an intermediate filament protein and is a marker for neural stem cells. Nestin is required for brain and eye development. Nestin expression generally doesn’t persist into adult hood, with the exception of the neuronal precursor cells of the subgranular zone. After cells have differentiated Nestin is downregulated and is replaced by tissue-specific intermediate filament proteins.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Nestin Capture Antibody 1 x 600µl 10X Mouse Nestin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse Nestin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells. -
Tissue specificity
CNS stem cells. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments. -
Post-translational
modificationsConstitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized. - Information by UniProt
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Alternative names
- ESTM 46
- FLJ 21841
- FLJ21841
see all -
Database links
- Entrez Gene: 18008 Mouse
- SwissProt: Q6P5H2 Mouse
- Unigene: 331129 Mouse
Associated products
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Alternative Versions
Images
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Other - Mouse Nestin ELISA Kit (ab223859)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of MouseNestin standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Nestin in Mouse NIH/3T3 cell extract sample and sample based on a 62.5 µg/mL extract loadThe concentrations of Nestin were measured in duplicate and interpolated from the Nestin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nestin concentration was determined to be 678 pg/mL in NIH/3T3 Cell extract.
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Sandwich ELISA - Mouse Nestin ELISA Kit (ab223859)To learn more about the advantages of recombinant antibodies see here.
References (0)
ab223859 has not yet been referenced specifically in any publications.
