• Product name

    Mouse Neurotrophin-3 ELISA Kit
    See all Neurotrophin 3 kits
  • Detection method

  • Precision

    Sample n Mean SD CV%
    Sample 1 16 88pg/ml 4.84 5.5%
    Sample 2 16 329pg/ml 19.74 6%
    Sample 3 16 682pg/ml 30.01 4.4%
    Sample n Mean SD CV%
    Sample 1 24 113pg/ml 7.12 6.3%
    Sample 2 24 395pg/ml 28.44 7.2%
    Sample 3 24 753pg/ml 39.16 5.2%
  • Sample type

    Cell culture supernatant, Serum
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 2 pg/ml
  • Range

    15.6 pg/ml - 1000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Mouse Neurotrophin-3 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213882) is designed for the quantitative measurement of Mouse Neurotrophin-3 in cell culture supernatants and serum.

    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for Neurotrophin-3 has been precoated onto 96-well plates. Standards (Expression system for standard: sf21; Immunogen sequence: Y139-T257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Neurotrophin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Neurotrophin-3 amount of sample captured in plate.

  • Notes

    Neurotrophin-3 (NT-3) is a new member of the nerve growth factor gene family, which plays an important role in the development and maintenance of the vertebrate nervous system. NT-3 and its receptor, called neurotrophic tyrosine kinase receptor type 3 (Ntrk3; also called TrkC), are expressed early and throughout embryogenesis. NT-3 is one of five neurotrophin growth factors which shape the development of the nervous system by regulating neuronal survival and differentiation. NT-3 may be one of the central nervous system-derived factors that mediate neural crest (NC) cell proliferation in vivo. NT-3 has been mapped to human chromosome 12p and mouse chromosome 6.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)


  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Mouse Neurotrophin-3 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Mouse Neurotrophin-3 antibody 1 x 130µl
    Lyophilized recombinant Mouse Neurotrophin-3 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Seems to promote the survival of visceral and proprioceptive sensory neurons.
  • Tissue specificity

    Brain and peripheral tissues.
  • Sequence similarities

    Belongs to the NGF-beta family.
  • Cellular localization

  • Information by UniProt
  • Alternative names

    • HDNF
    • MGC129711
    • Nerve growth factor 2
    • Neurotrophic factor
    • Neurotrophin 3 precursor
    • Neurotrophin-3
    • Neurotrophin3
    • NGF 2
    • NGF-2
    • NGF2
    • NT 3
    • NT-3
    • NT3
    • NTF 3
    • NTF3
    • NTF3_HUMAN
    see all
  • Database links


Our Abpromise guarantee covers the use of ab213882 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Mouse Neurotrophin-3 ELISA Kit (ab213882) Standard Curve.



ab213882 has not yet been referenced specifically in any publications.

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