Overview

  • Product name

    Mouse NGF R ELISA Kit (CD271)
    See all NGFR kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 2.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 1.8%
  • Sample type

    Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    1.6 pg/ml
  • Range

    11 pg/ml - 700 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 110 104% - 114%
    Heparin Plasma 112 101% - 120%
    EDTA Plasma 104 99% - 108%
    Citrate Plasma 106 102% - 108%
    Mouse brain extract 109 107% - 113%

  • Assay time

    1h 30m
  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Mouse, Rat
    Does not react with: Rabbit, Goat, Guinea pig, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s NGF R (CD271) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of NGF R protein in mouse serum, plasma, cell and tissue extracts.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

    Sensitivity:

    Samples diluted in Sample Diluent NS: 2.4 pg/mL

    Samples diluted in 1X Cell Extraction Buffer PTR: 1.6 pg/mL

  • Notes

    NGF R is a receptor involved in neuronal cell death and cell survival, insulin mediated glucose uptake in adipocytes and skeletal muscle, metabolism in the liver, and in circadian rhythms.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse NGF R Capture Antibody 1 x 600µl
    10X Mouse NGF R Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BR 1 x 6ml
    Mouse NGF R Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas

  • Relevance

    Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver.
  • Cellular localization

    Membrane; Single-pass type I membrane protein.
  • Alternative names

    • CD271
    • Gp80-LNGFR
    • Low affinity neurotrophin receptor p75NTR
    • Low-affinity nerve growth factor receptor
    • Nerve growth factor receptor
    • NGF receptor
    • NGFR
    • p75 ICD
    • p75(NTR)
    • p75NTR
    • TNFR superfamily member 16
    • TNFRSF16
    • Tumor necrosis factor receptor superfamily member 16
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab205569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of mouse NGF R were measured in duplicate and interpolated from the mouse NGF R standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

  • The concentrations of mouse NGF R were interpolated from the mouse NGF R standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted. Interpolated values of NGF R are plotted based on an extract load of 500 µg/mL.

Protocols

References

ab205569 has not yet been referenced specifically in any publications.

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