Mouse NMDAR1 ELISA Kit (ab282862)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 27.74 pg/ml
- Range: 203.13 pg/ml - 13000 pg/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse NMDAR1 ELISA Kit
See all NMDAR1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 2.9% Inter-assay Sample n Mean SD CV% Extract 3 3.8% -
Sample type
Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
27.74 pg/ml -
Range
203.13 pg/ml - 13000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 92 80% - 100% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Human NMDAR1 ELISA kit (ab282862) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human NMDAR1 protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human NMDAR1 with 27.74 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mouse NMDA receptor (NMDAR1) is a glutamate and ion channel protein receptor activated upon glycine and glutamate binding. NMDAR1 is a component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. NMDA receptor complex channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition. Mouse NMDAR1 shares 99.8% and 99.3% sequence homology with rat and human, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse NMDAR1 Capture Antibody 1 x 600µl 10X Mouse NMDAR1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse NMDAR1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. -
Post-translational
modificationsNMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities. - Information by UniProt
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Alternative names
- GluN1
- Glutamate [NMDA] receptor subunit zeta-1
- Glutamate receptor ionotropic N methyl D aspartate 1
see all -
Database links
- Entrez Gene: 14810 Mouse
- SwissProt: P35438 Mouse
- Unigene: 278672 Mouse
Associated products
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Alternative Versions
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody BenefitsTo learn more about the advantages of recombinant antibodies see here.
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Example of mouse NMDAR1 standard curve in 1X Cell Extraction Buffer PTR.The NMDAR1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native NMDAR1 in mouse brain tissue extract based on a 125 µg/mL extract load.The concentrations of NMDAR1 were measured in duplicate and interpolated from the NMDAR1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NMDAR1 concentration was determined to be 7515.05 pg/mL in brain tissue extract.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab282862 has not yet been referenced specifically in any publications.